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Product Info Summary
| SKU: | M05349-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-SET07 Antibody
SKU/Catalog Number
M05349-1
Size
80 µl
Description
Boster Bio Anti-SET07 Antibody (Catalog # M05349-1). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SET07 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05349-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
43AT551.86
Isotype
Mouse IgG1
Immunogen
This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
Reactive Species
M05349-1 is reactive to KMT5A in Human
Calculated molecular weight
42.9 kDa
Background of KMT5A
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05349-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF: 1:10-1:50
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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All lanes :SET07 Antibody at 1:2000 dilution
Lane 1: 293T whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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(TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).
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Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-SET07 Antibody (M05349-1)
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