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Product Info Summary
| SKU: | LS064135 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of interferon induced with helicase C domain 1 (IFIH1) |
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Product info
Product Name
MDA5 (IFIH1) (NM_022168) Human Over-expression Lysate
SKU/Catalog Number
LS064135
Size
100 µg
Description
Transient overexpression lysate of interferon induced with helicase C domain 1 (IFIH1)
Storage & Handling
Cite This Product
MDA5 (IFIH1) (NM_022168) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064135)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MDA5 (IFIH1) Human Over-expression Lysates NM_022168.
Western blot validation of overexpression lysate (LS064135) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IFIH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IFIH1 (Source: Uniprot.org, NCBI)
Gene Name
IFIH1
Full Name
Interferon-induced helicase C domain-containing protein 1
Weight
116.689kDa
Superfamily
helicase family
Alternative Names
CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; EC 3.6.4.13; helicard; Helicase with 2 CARD domains; Hlcd; IDDM19; interferon induced with helicase C domain 1; interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5MGC133047; MDA5RNA helicase-DEAD box protein 116; melanoma differentiation associated protein-5; Melanoma differentiation-associated protein 5; Murabutide down-regulated protein; RH116 IFIH1 AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1 interferon induced with helicase C domain 1 interferon-induced helicase C domain-containing protein 1|CADM-140 autoantigen|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis autoantigen 140 kDa|helicard|helicase with 2 CARD domains|melanoma differentiation-associated gene 5|melanoma differentiation-associated protein 5|murabutide down-regulated protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IFIH1, check out the IFIH1 Infographic
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In this infographic, you will see the following information for IFIH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MDA5 (IFIH1) (NM_022168) Human Over-expression Lysate (LS064135)
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