Anti-MDA5 IFIH1 Antibody

Product Name

Anti-MDA5 IFIH1 Antibody

View all IFIH1 Antibodies

SKU/Catalog Number

A00263

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MDA5 IFIH1 Antibody (Catalog # A00263). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

MDA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MDA5 IFIH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00263)

Host

Rabbit

Contents

MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5.

Reactive Species

A00263 is reactive to IFIH1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

116.7 kDa

Background of IFIH1

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00263 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information IFIH1

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