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Product Info Summary
| SKU: | M00263-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-MDA5 Monoclonal Antibody
SKU/Catalog Number
M00263-1
BM5399 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MDA5 Monoclonal Antibody catalog # M00263-1. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00263-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADDD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MDA5
Reactive Species
M00263-1 is reactive to IFIH1 in Human
Observed Molecular Weight
117 kDa
Calculated molecular weight
116.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00263-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: THP1 cell
Validation Images & Assay Conditions
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Western blot analysis of MDA5 expression in THP1 cell lysate.
Specific Publications For Anti-MDA5 Monoclonal Antibody (M00263-1)
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4 Customer Q&As for Anti-MDA5 Monoclonal Antibody
Question
We are currently using anti-MDA5 Monoclonal antibody M00263-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-MDA5 Monoclonal antibody (M00263-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this M00263-1 anti-MDA5 Monoclonal antibody reactive to the isotypes of IFIH1?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
The immunogen of M00263-1 anti-MDA5 Monoclonal antibody is A synthesized peptide derived from human MDA5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-07
Question
Will anti-MDA5 Monoclonal antibody M00263-1 work on zebrafish WB with spleen?
K. Parker
Verified customer
Asked: 2017-02-08
Answer
Our lab technicians have not validated anti-MDA5 Monoclonal antibody M00263-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-MDA5 Monoclonal antibody M00263-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-08
Question
I was wanting to use your anti-MDA5 Monoclonal antibody for WB for human parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human parotid gland identification?
T. Banerjee
Verified customer
Asked: 2013-07-01
Answer
You can see on the product datasheet, M00263-1 anti-MDA5 Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-01
