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Description
Mix-n-Stain™ Nanobody Labeling Kits are the only kits specifically designed for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with fluorescent dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. The kit reduces the chance of over-labeling the nanobody, which can affect antigen binding. Labeling tolerates common buffer additives, including BSA. The kits come with a selection of next-generation fluorescent CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. These kits allow labeling of 5-20 ug or 20-50 ug of Nanobody®, with a choice of 7 CF® Dye colors or biotin. They feature simple 30 minute labeling with no purification, compatibility with common stabilizers, and are designed to prevent over-labeling. Dye options are available for super-resolution and 2-photon imaging. Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, users are advised to consult the product protocol to review kit compatibility and protocol selection guidelines. Biotium also offers a Mix-n-Stain™ Nanobody Thiol Labeling Kit designed for labeling VHH via a single cysteine only, which is useful for labeling commercially available nanobodies that contain a single cysteine or for labeling VHH for functionality studies. For labeling IgG antibodies, Mix-n-Stain™ Antibody Labeling Kits are recommended. Mix-n-Stain™ Small Ligand Labeling Kits are available for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. The Mix-n-Stain™ product line includes a full selection of antibody, protein, and ligand labeling kits to support a wide range of fluorescence labeling applications.
Details
Mix-n-Stain™ Nanobody Labeling Kits are specifically optimized for labeling single-domain Nanobodies® (camelid single variable or VHH domains) with fluorescent CF® Dyes or biotin. The kits are designed to label 5–50 µg of nanobody in approximately 30 minutes with minimal hands-on time and no post-labeling purification.
- Optimized for 5–20 µg or 20–50 µg of Nanobody®
- Choice of 7 CF® Dye colors or biotin
- Simple 30 minute workflow with no purification step
- Formulation helps prevent over-labeling that can compromise antigen binding
- Compatible with common stabilizers, including BSA
- Dye options suitable for super-resolution and 2-photon imaging
CF® Dyes offer high brightness and photostability compared to many commonly used fluorescent dyes, making these kits suitable for demanding fluorescence microscopy and flow cytometry applications.
| Label | Ex/Em | Labeling size (µg Nanobody®) | Catalog number |
|---|---|---|---|
| Biotin | N/A | 5–20 µg | 92500 |
| Biotin | N/A | 20–50 µg | 92501 |
| CF®405S | 404/431 nm | 5–20 µg | 92502 |
| CF®405S | 404/431 nm | 20–50 µg | 92503 |
| CF®488A | 490/515 nm | 5–20 µg | 92504 |
| CF®488A | 490/515 nm | 20–50 µg | 92505 |
| CF®568 | 562/583 nm | 5–20 µg | 92506 |
| CF®568 | 562/583 nm | 20–50 µg | 92507 |
| CF®594 | 593/614 nm | 5–20 µg | 92508 |
| CF®594 | 593/614 nm | 20–50 µg | 92509 |
| CF®640R | 642/662 nm | 5–20 µg | 92510 |
| CF®640R | 642/662 nm | 20–50 µg | 92511 |
| CF®647 | 650/665 nm | 5–20 µg | 92512 |
| CF®647 | 650/665 nm | 20–50 µg | 92513 |
| CF®680R | 680/701 nm | 5–20 µg | 92514 |
| CF®680R | 680/701 nm | 20–50 µg | 92515 |
Before choosing a kit, users should review the product protocol for detailed kit compatibility and protocol selection guidance. Related Mix-n-Stain™ product lines are available for IgG antibodies, nanobodies with a single cysteine, and small ligands such as SNAP-tag®, CLIP-tag™, and HaloTag® substrates.
Conjugation Options
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About the Bundle and Save Program
Bundle and Save brings curated products from partner manufacturers into one streamlined catalog — you order through Boster, the partner ships directly to your lab. Skip the multi-vendor procurement overhead and unlock bundle pricing.
- One PO, one invoice — consolidated through Boster
- Pre-negotiated savings vs ordering direct from the manufacturer
- Direct manufacturer fulfillment — faster than transshipment
- Boster customer support handles the partner liaison
Cite This Product
Mix-n-Stain™ Nanobody Labeling Kits (Boster Biological Technology, Pleasanton CA, USA, Catalog # bs-biotium-92500)
