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Description
Product Description
Mix-n-Stain™ Nanobody Thiol Labeling Kits are designed for labeling VHH (also known as Nanobody® or single-domain antibodies) that contain a single free cysteine. The labeling is done within 2 hours in ambient conditions, and requires minimal hands-on time and no purification.
Note: This kit is for labeling VHH via a single cysteine (1x Cys) only. Please see our Mix-n-Stain™ Nanobody Labeling Kits for standard labeling of VHH, or view our full selection of labeling kits for labeling IgG antibodies or other biomolecules.
Features
- Label 5-20 ug or 20-50 ug VHH with single exposed cysteine residue
- Choice of 7 CF® Dye colors
- Simple 2 hour labeling, no purification
- No purification, 100% recovery
Kit components
- Lyophilized Reactive CF® Dye
- Mix-n-Stain™ Reaction Buffer, 5X
- Mix-n-Stain™ Activating Agent
- Mix-n-Stain™ Quenching Buffer, 10X
- Mix-n-Stain™ Storage Buffer, 4X
Superior CF® Dyes
These kits feature a choice of Biotium's next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.
Choosing the Right Kit for Your Application
Before selecting a Mix-n-Stain™ Nanobody Thiol Labeling Kit, please download the Product Protocol to see our Kit Compatibility guidelines.
For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.
Mix-n-Stain™ Nanobody Thiol Labeling Kits
| Conjugation | Ex/Em | Labeling size (ug Nanobody®) | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®488A | 490/516 nm | 5-20 ug | 92585 | CF®488A Features |
| 20-50 ug | 92586 | |||
| CF®568 | 562/584 nm | 5-20 ug | 92587 | CF®568 Features |
| 20-50 ug | 92588 | |||
| CF®583R | 585/609 nm | 5-20 ug | 92589 | CF®583R Features |
| 20-50 ug | 92590 | |||
| CF®594 | 593/615 nm | 5-20 ug | 92591 | CF®594 Features |
| 20-50 ug | 92592 | |||
| CF®647 | 652/668 nm | 5-20 ug | 92593 | CF®647 Features |
| 20-50 ug | 92594 | |||
| CF®680 | 681/698 nm | 5-20 ug | 92595 | CF®680 Features |
| 20-50 ug | 92596 | |||
| CF®740 | 742/767 nm | 5-20 ug | 92597 | CF®740 Features |
| 20-50 ug | 92598 |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
| Mix-n-Stain™ CF® Dye Antibody Labeling Kits | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
| Mix-n-Stain™ FITC Antibody Labeling Kits | FITC | |||
| Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits | Cyanine 555 Cyanine 647 |
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| Mix-n-Stain™ Biotin Antibody Labeling Kits | Biotin | |||
| Mix-n-Stain™ Digoxigenin Antibody Labeling Kits | Digoxygenin (DIG) | |||
| Mix-n-Stain™ DNP Antibody Labeling Kits | DNP | |||
| Mix-n-Stain™ Maxi Antibody Labeling Kits | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
| Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
| Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
| Mix-n-Stain™ Nanobody Labeling Kits | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
| Mix-n-Stain™ Nanobody Thiol Labeling Kits | CF® Dyes | ~ 2 hours | • Optimized for Nanobodies® with single exposed cysteine residue | |
| Mix-n-Stain™ Small Ligand Labeling Kits | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
| VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
| CF® Dye & Biotin SE Protein Labeling Kits | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
Details
Documents
Conjugation Options
| Label | Feature | Pack | Catalog # | Price (USD) |
|---|---|---|---|---|
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About the Bundle and Save Program
Bundle and Save brings curated products from partner manufacturers into one streamlined catalog — you order through Boster, the partner ships directly to your lab. Skip the multi-vendor procurement overhead and unlock bundle pricing.
- One PO, one invoice — consolidated through Boster
- Pre-negotiated savings vs ordering direct from the manufacturer
- Direct manufacturer fulfillment — faster than transshipment
- Boster customer support handles the partner liaison
Cite This Product
Mix-n-Stain™ Nanobody Thiol Labeling Kits (Boster Biological Technology, Pleasanton CA, USA, Catalog # bs-biotium-92585)
