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Description
Product Description
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits allow you to label 50 ug of your antibody with one of Biotium's STORM CF® Dyes to produce an antibody conjugate with a low 1-2.5 DOL (degree of labeling) optimal for super-resolution STORM (Stochastic Optical Reconstruction Microscopy). Labeling takes just 30 minutes, with minimal hands-on time and no purification.
Features
- Label 50 ug antibody
- Choice of 8 CF® Dye colors ideal for STORM
- CF®583R and CF®597R are novel green-excited STORM dyes
- Labeling optimized to provide low 1-2.5 DOL
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive STORM CF® Dye
- Mix-n-Stain™ Storage Buffer
Superior CF® Dyes for STORM Imaging
These kits feature a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (1). Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.
Discover More with Green-Excitable CF® Dyes Developed for Multi-Color STORM
High-quality STORM imaging relies heavily on bright fluorescent dyes with excellent photoswitching properties. While there are several commonly used red-excited dyes suitable for STORM imaging such as CF®647, CF®660C and CF®680, generating comparable dyes for other excitation sources to produce quality multi-color STORM images remains a challenge. Biotium scientists have met this challenge by developing novel chemical modifications for green-excited fluorescent dyes to offer optimal performance for STORM super-resolution imaging . This includes CF®583R and CF®597R which were developed specifically for STORM imaging and display exceptional performance for multi-color STORM (2).
Two-color (d)STORM imaging of a fixed COS-7 cell: microtubules were immunolabeled with CF®647 conjugate, and mitochondria were immunolabeled with CF®583R conjugate. (d)STORM was performed under standard conditions using a standard tris-based (d)STORM imaging buffer containing 100 mM cysteamine and an oxygen scavenger. CF®647 was first imaged with 647 nm excitation, and CF®583R was next imaged with 561 nm excitation. Images courtesy of Bowen Wang, Michael Xiong, and Professor Ke Xu, College of Chemistry, University of California, Berkeley.
See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye. Select the links in the Dye Features column to learn more about each CF® Dye and potential applications.
Other Labeling Kits
We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®505 | 505/519 nm | 50 ug | 92549 | |
| CF®568 | 562/584 nm | 50 ug | 92550 | CF®568 Features |
| CF®583R | 585/609 nm | 50 ug | 92551 | CF®583R Features |
| CF®597R | 597/619 nm | 50 ug | 92553 | CF®597R Features |
| CF®647 | 652/668 nm | 50 ug | 92554 | CF®647 Features |
| CF®660C | 667/685 nm | 50 ug | 92555 | CF®660C Features |
| CF®680 | 681/698 nm | 50 ug | 92556 | CF®680 Features |
| CF®750 | 755/779 nm | 50 ug | 92557 | CF®750 Features |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
| Mix-n-Stain™ CF® Dye Antibody Labeling Kits | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
| Mix-n-Stain™ FITC Antibody Labeling Kits | FITC | |||
| Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits | Cyanine 555 Cyanine 647 |
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| Mix-n-Stain™ Biotin Antibody Labeling Kits | Biotin | |||
| Mix-n-Stain™ Digoxigenin Antibody Labeling Kits | Digoxygenin (DIG) | |||
| Mix-n-Stain™ DNP Antibody Labeling Kits | DNP | |||
| Mix-n-Stain™ Maxi Antibody Labeling Kits | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
| Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
| Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
| Mix-n-Stain™ Nanobody Labeling Kits | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
| Mix-n-Stain™ Nanobody Thiol Labeling Kits | CF® Dyes | ~ 2 hours | • Optimized for Nanobodies® with single exposed cysteine residue | |
| Mix-n-Stain™ Small Ligand Labeling Kits | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
| VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
| CF® Dye & Biotin SE Protein Labeling Kits | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
CF is a registered trademark of Biotium, Inc.
Details
Documents
Conjugation Options
| Label | Feature | Pack | Catalog # | Price (USD) |
|---|---|---|---|---|
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About the Bundle and Save Program
Bundle and Save brings curated products from partner manufacturers into one streamlined catalog — you order through Boster, the partner ships directly to your lab. Skip the multi-vendor procurement overhead and unlock bundle pricing.
- One PO, one invoice — consolidated through Boster
- Pre-negotiated savings vs ordering direct from the manufacturer
- Direct manufacturer fulfillment — faster than transshipment
- Boster customer support handles the partner liaison
Cite This Product
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits (Boster Biological Technology, Pleasanton CA, USA, Catalog # bs-biotium-92549)
