MKP-1/2 (Phospho-Ser296/318) Colorimetric Cell-Based ELISA Kit

Product Name

MKP-1/2 (Phospho-Ser296/318) Colorimetric Cell-Based ELISA Kit

See all DUSP1 CL100 , MKP1, PTPN10 , VH1 products

SKU/Catalog Number

EKC2619

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The MKP-1/2 (Phospho-Ser296/318) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MKP-1/2 (Phospho-Ser296/318) protein expression profile in cells. The kit can be used for measuring the relative amounts of MKP-1/2 (Phospho-Ser296/318) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MKP-1/2 (Phospho-Ser296/318).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

MKP-1/2 (Phospho-Ser296/318) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2619)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2619 is reactive to DUSP1 CL100 , MKP1, PTPN10 , VH1 in Human, Mouse, Rat samples

Application Guarantee

EKC2619 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	2 Plates	-
10x TBS	30 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	60 ml (15x)	Clear
100x Anti-MKP-1/2 Phospho-Ser296/318 Antibody (Rabbit Polyclonal)	60 µl (100x)	Red
100x Anti-MKP-1/2 Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	12ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	12ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	12ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For DUSP1 CL100 , MKP1, PTPN10 , VH1 (Source: Uniprot.Org, NCBI)

Alternative Names

Dual specificity protein phosphatase 1 ;3.1.3.16 ;3.1.3.48 ;Dual specificity protein phosphatase hVH1 ;Mitogen-activated protein kinase phosphatase 1;MAP kinase phosphatase 1;MKP-1;Protein-tyrosine phosphatase CL100 ;DUSP1 ;CL100 , MKP1, PTPN10 , VH1 ;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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