Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine®

sFRP-5 ELISA kit for Monkey

Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey SFRP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Product Info Summary

SKU: EK1472-PR
Size: 96 wells/kit, with removable strips.
Reactive Species: Monkey
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

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Product Name

Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine®

View all sFRP-5 ELISA kits

SKU/Catalog Number

EK1472-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey SFRP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1472-PR)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: E30-H317

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1472-PR is reactive to SFRP5 in Monkey samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1472-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of sFRP-5

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Kit Components

Catalog Number Description Quantity
EK1472-PR-CAP Anti-Monkey SFRP5 Pre-coated 96-well strip microplate 1
EK1472-PR-ST Monkey SFRP5 Standard 2 vials, 10 ng/tube
EK1472-PR-DA Monkey SFRP5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0560.1680.2940.5160.9531.4871.9122.189

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7492783602587729487356
Standard deviation53.93178.11439.8274.54256.47698.82
CV (%)7.26.47.38.58.79.5

Gene/Protein Information For SFRP5 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q5T4F7

Gene ID

6425

Gene Name

SFRP5

Full Name

Secreted frizzled-related protein 5

Weight

35.563kDa

Superfamily

secreted frizzled-related protein (sFRP) family

Alternative Names

frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5 SFRP5 SARP3 secreted frizzled related protein 5 secreted frizzled-related protein 5|FRP-1b|SARP-3|frizzled-related protein 1b|sFRP-5|secreted apoptosis related protein 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SFRP5, check out the SFRP5 Infographic

SFRP5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SFRP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine®

Question

Q: how can I thaw whole blood sample for sFRP-5 ELISA after freezing?

D. King

Verified customer

Asked: 2020-11-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test sFRP-5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-11-06

Question

Q: can I use citrate plasma as samples in Monkey sFRP-5 Picokine® ELISA Kit (Catalog # EK1472-PR)?

V. Edwards

Verified customer

Asked: 2018-12-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of sFRP-5 causing disruption of its protein structure. sFRP-5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the sFRP-5 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-23

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

P. Surve

Verified customer

Asked: 2018-11-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-11-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

R. Banerjee

Verified customer

Asked: 2018-10-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-22

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-08-12

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-08-12

Question

Q: how to analyze ELISA data? I have obtained sFRP-5 level in plasma.

A. Johnson

Verified customer

Asked: 2018-05-27

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-27

Question

Q: if the enzyme conjugated sFRP-5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the sFRP-5 antigen?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-29

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring sFRP-5 using the EK1472-PR Monkey sFRP-5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-03-03

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2015-03-03

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