Mouse ADAM12 ELISA Kit PicoKine™

Mouse ADAM12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Adam12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1294
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse ADAM12 ELISA Kit PicoKine™

See all Adam12 products

SKU/Catalog Number

EK1294

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse ADAM12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Adam12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse ADAM12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1294)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E206-G706

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1294 is reactive to Adam12 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1294 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1294-CAP Anti-Mouse Adam12 Pre-coated 96-well strip microplate 1
EK1294-ST Mouse Adam12 Standard 2 vials, 10 ng/tube
EK1294-DA Mouse Adam12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0230.0490.0910.1520.2910.5610.9641.823

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Adam12, Dilution ratio of 1:1, concentration in serum is 176-208pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse ADAM12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2641864480224518314402
Standard deviation11.35147.25312.1314.94179.43369.76
CV (%)4.3%7.9%6.5%6.1%9.8%8.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adam12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 126431328329028717.526.1%
Sample 21864176019221772182966.863.6%
Sample 348024607472145104660110.892.3%
*number of samples for each test n=16.

Gene/Protein Information For Adam12 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q61824

Gene ID

11489

Gene Name

Adam12

Full Name

Disintegrin and metalloproteinase domain-containing protein 12

Weight

98.504kDa

Alternative Names

a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.- Adam12|ADAM, Ml, Mltna|a disintegrin and metallopeptidase domain 12 (meltrin alpha)|disintegrin and metalloproteinase domain-containing protein 12|a disintegrin and metalloprotease domain 12|a disintegrin and metalloproteinase domain 12 (meltrin alpha)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Adam12, check out the Adam12 Infographic

Adam12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Adam12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse ADAM12 ELISA Kit PicoKine™

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-10-04

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-10-04

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-12-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-12-08

Question

Q: which procedure should I follow in order to thaw whole blood sample for ADAM12 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-03-13

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ADAM12 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-03-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

P. Liu

Verified customer

Asked: 2019-01-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-28

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-08-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-08-14

Question

Q: how to analyze ELISA data? I measured ADAM12 level in serum.

W. Williams

Verified customer

Asked: 2018-01-27

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-27

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