Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

Mouse Adipsin / CFD / Factor D ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cfd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK1420
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

See all Complement Factor D/Adipsin products

SKU/Catalog Number

EK1420

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Adipsin / CFD / Factor D ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cfd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1420)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I26-S259

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1420 is reactive to Cfd in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1420 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1420-CAP Anti-Mouse Cfd Pre-coated 96-well strip microplate 1
EK1420-ST Mouse Cfd Standard 2 vials, 50 ng/tube
EK1420-DA Mouse Cfd Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.1310.2110.2420.3550.5350.8891.4662.017

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Complement Factor D/Adipsin, Dilution ratio of 1:20000, concentration in serum is 93-260ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14354423194181328478318803
Standard deviation107.62309.611378.67111.55411.331541.84
CV (%)7.5%7%7.1%8.4%8.6%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Factor D/Adipsin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11435148215541340145277.675.3%
Sample 244234063408239524130176.294.2%
Sample 31941820714181372028919639985.265%
*number of samples for each test n=16.

Gene/Protein Information For Cfd (Source: Uniprot.Org, NCBI)

Uniprot ID

P03953

Gene ID

11537

Gene Name

Cfd

Full Name

Complement factor D

Weight

28.057kDa

Superfamily

peptidase S1 family

Alternative Names

Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN Cfd|A, Adn, DF|complement factor D (adipsin)|complement factor D|28 kDa adipocyte protein|C3 convertase activator|D component (adipsin) of complement|properdin factor D

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cfd, check out the Cfd Infographic

Cfd infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cfd: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Reviews For Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

0

"Pretty good product with a reasonable price"

Excellent

Source: Biocompare.com

"Pretty good product with a reasonable price"

--Xiaobing Cui, University of Georgia, Physiology & Pharmacology, Research Scientist

Application ELISA
Sample Serum and supernatant
Detection --

"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")

Verified customer

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8 Customer Q&As for Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-09-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-09-18

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-03-27

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Complement Factor D using the EK1420 Mouse Complement Factor D Picokine® ELISA Kit?

R. Taylor

Verified customer

Asked: 2019-04-20

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-04-20

Question

Q: how to thaw whole blood sample for Complement Factor D ELISA after freezing?

Y. Thomas

Verified customer

Asked: 2019-02-09

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Complement Factor D for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-02-09

Question

Q: What is the optimal O.D. value for Complement Factor D ELISA kit? I performed your Complement Factor D ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Complement Factor D even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-08-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Complement Factor D ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of Complement Factor D that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2018-08-27

Question

Q: Can Complement Factor D ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

A. Walker

Verified customer

Asked: 2018-04-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-04-10

Question

Q: how to proceed with the analysis of ELISA data? I have performed Complement Factor D level in serum.

V. Hill

Verified customer

Asked: 2017-12-21

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-12-21

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