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Product Info Summary
| SKU: | EK2036 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse ANGPTL7 ELISA Kit PicoKine®
SKU/Catalog Number
EK2036
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse ANGPTL7 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ANGPTL7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2036)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q22-P337
Capture/Detection Antibodies
a polyclonal antibody from goat|a polyclonal antibody from goat
Sensitivity
<15 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2036 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2036 is reactive to Angptl7 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2036 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angptl7
Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 4; 4 E2. Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2036-CAP | Anti-Mouse Angptl7 Pre-coated 96-well strip microplate | 1 |
| EK2036-ST | Mouse Angptl7 Standard | 2 vials, 10 ng/tube |
| EK2036-DA | Mouse Angptl7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.063 | 0.109 | 0.165 | 0.254 | 0.459 | 0.722 | 1.273 | 2.084 |
Data Example Images
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Mouse ANGPTL7 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angptl7, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ANGPTL7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 143 | 496 | 1744 | 117 | 390 | 1677 |
| Standard deviation | 7.32 | 17.26 | 107.78 | 9.08 | 20.20 | 99.45 |
| CV (%) | 5.1% | 3.5% | 6.2% | 7.8% | 5.2% | 5.9% |
Specific Publications For Mouse ANGPTL7 ELISA Kit PicoKine® (EK2036)
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Customer Reviews
0 Reviews For Mouse ANGPTL7 ELISA Kit PicoKine®
Customer Q&As
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12 Customer Q&As for Mouse ANGPTL7 ELISA Kit PicoKine®
Question
What is the concentration of Sulfuric acid and Sodium azide in EK2036?
Verified customer
Asked: 2022-03-23
Answer
The stop solution of Mouse ANGPTL7 ELISA Kit PicoKine® (EK2036) contains 2%.
Boster Scientific Support
Answered: 2022-03-24
Question
If a recombinant protein such as standard or coating antigen is included in EK2036, what is the expression species of the protein and was viral vectors used?
Verified customer
Asked: 2022-03-23
Answer
The standard in Mouse ANGPTL7 ELISA Kit PicoKine® (EK2036) is a recombinant protein. Expression system is CHO. No viral vectors are used.
Boster Scientific Support
Answered: 2022-03-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis ANGPTL7 using the EK2036 Mouse ANGPTL7 Picokine® ELISA Kit?
B. Kulkarni
Verified customer
Asked: 2020-08-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-29
Question
Has EK2036 been tested for cross-reactivity with related proteins, such as ANGPT1,2,4 and ANGPTL1-6 and 8?
Verified customer
Asked: 2020-03-11
Answer
The Mouse ANGPTL7 ELISA Kit PicoKine EK2036 hasn't been tested for cross-reactivity with related proteins such as ANGPT1,2,4 and ANGPTL1-6 and 8.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
H. Thomas
Verified customer
Asked: 2020-02-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Kulkarni
Verified customer
Asked: 2019-11-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-11-15
Question
What is the host for the capture and detection antibody of EK2036?
Verified customer
Asked: 2019-10-30
Answer
The host for the capture and detection antibody of the Mouse ANGPTL7 ELISA Kit PicoKine™ (EK2036) is Goat Polyclonal.
Boster Scientific Support
Answered: 2019-10-31
Question
What is the purification medthod for the capture and detection antibody of EK2036?
Verified customer
Asked: 2019-10-30
Answer
The purification method for the capture and detection antibody of the Mouse ANGPTL7 ELISA Kit PicoKine™ (EK2036) is Affinity Purified.
Boster Scientific Support
Answered: 2019-10-31
Question
What is the standard source and expression for EK2036?
Verified customer
Asked: 2019-10-30
Answer
For the Mouse ANGPTL7 ELISA Kit PicoKine™ (EK2036), the standard source is recombinant protein, expression is CHO Expression System.
Boster Scientific Support
Answered: 2019-10-31
Question
What is the immunogen for the capture antibody of EK2036?
Verified customer
Asked: 2019-10-30
Answer
The immunogen for the capture antibody of the Mouse ANGPTL7 ELISA Kit PicoKine™ (EK2036) is Q22-P337.
Boster Scientific Support
Answered: 2019-10-31
Question
Q: Can ANGPTL7 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: how to analyze ELISA data? I measured ANGPTL7 level in serum.
L. Scott
Verified customer
Asked: 2016-06-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2016-06-26
