Mouse BMP-6 ELISA Kit PicoKine™

Mouse BMP-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmp6 in bone tissue, cell culture supernatants and serum. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1608
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: bone tissue, cell culture supernatants and serum.

Product Name

Mouse BMP-6 ELISA Kit PicoKine™

See all BMP-6 products

SKU/Catalog Number

EK1608

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse BMP-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmp6 in bone tissue, cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse BMP-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1608)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: S372-H510

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1608 is reactive to Bmp6 in Mouse samples

Validated Sample Types

bone tissue, cell culture supernatants and serum.

Application Guarantee

EK1608 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1608-CAP Anti-Mouse Bmp6 Pre-coated 96-well strip microplate 1
EK1608-ST Mouse Bmp6 Standard 2 vials, 10 ng/tube
EK1608-DA Mouse Bmp6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.1130.1720.2260.3470.5470.9571.5332.373

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse BMP-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2141829579022616735512
Standard deviation12.4174.98399.5113.3386.99391.35
CV (%)5.8%4.1%6.9%5.9%5.2%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BMP-6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121420824722622314.96.6%
Sample 21829183518241804182311.640.6%
Sample 357906282615859296039191.93.1%
*number of samples for each test n=16.

Gene/Protein Information For Bmp6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20722

Gene ID

12161

Gene Name

Bmp6

Full Name

Bone morphogenetic protein 6

Weight

56.432kDa

Superfamily

TGF-beta family

Alternative Names

BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1 Bmp6|D13Wsu115, D13Wsu115e, Vg, Vgr1|bone morphogenetic protein 6|bone morphogenetic protein 6|VG-1-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bmp6, check out the Bmp6 Infographic

Bmp6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bmp6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse BMP-6 ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have performed BMP-6 level in bone tissue.

Verified Customer

Verified customer

Asked: 2020-11-07

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-11-07

Question

Q: can I use EDTA plasma as samples in Mouse BMP-6 Picokine® ELISA Kit (Catalog # EK1608)?

B. Martin

Verified customer

Asked: 2020-06-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of BMP-6 causing degradation of its protein structure. BMP-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the BMP-6 ELISA, treating samples with a number of anticoagulants and decided that / can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-06-09

Question

Have you tried mouse sera to detect bmp-6 using Mouse BMP-6 ELISA Kit PicoKine?

Verified customer

Asked: 2019-11-21

Answer

Our laboratory technicains obtained very low sample values by working on normal mouse sera samples which contains little BMP-6. The kit will work for some pathological sera samples in which the expression level of BMP-6 is high.

Boster Scientific Support

Answered: 2019-11-26

Question

Q: if the enzyme conjugated BMP-6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-6 antigen?

Verified Customer

Verified customer

Asked: 2019-02-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-02-23

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-02-01

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-15

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-10-15

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-09-29

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