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Product Info Summary
| SKU: | EK1608 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants and serum. |
Product info
Product Name
Mouse BMP-6 ELISA Kit PicoKine®
SKU/Catalog Number
EK1608
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse BMP-6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bmp6 in bone tissue, cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BMP-6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1608)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: S372-H510
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1608 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1608 is reactive to Bmp6 in Mouse samples
Validated Sample Types
bone tissue, cell culture supernatants and serum.
Application Guarantee
EK1608 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Bmp6
Bone morphogenetic protein 6 is a protein that in humans is encoded by the BMP6 gene. It is mapped to 6p24-p23. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1608-CAP | Anti-Mouse Bmp6 Pre-coated 96-well strip microplate | 1 |
| EK1608-ST | Mouse Bmp6 Standard | 2 vials, 10 ng/tube |
| EK1608-DA | Mouse Bmp6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.052 | 0.106 | 0.159 | 0.249 | 0.438 | 0.781 | 1.515 | 2.342 |
Data Example Images
Click image to see more details
Mouse BMP-6 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BMP-6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 214 | 1829 | 5790 | 226 | 1673 | 5512 |
| Standard deviation | 12.41 | 74.98 | 399.51 | 13.33 | 86.99 | 391.35 |
| CV (%) | 5.8% | 4.1% | 6.9% | 5.9% | 5.2% | 7.1% |
Specific Publications For Mouse BMP-6 ELISA Kit PicoKine® (EK1608)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse BMP-6 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have performed BMP-6 level in bone tissue.
Verified Customer
Verified customer
Asked: 2020-11-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-11-07
Question
Q: can I use EDTA plasma as samples in Mouse BMP-6 Picokine® ELISA Kit (Catalog # EK1608)?
B. Martin
Verified customer
Asked: 2020-06-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of BMP-6 causing degradation of its protein structure. BMP-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the BMP-6 ELISA, treating samples with a number of anticoagulants and decided that / can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-09
Question
Have you tried mouse sera to detect bmp-6 using Mouse BMP-6 ELISA Kit PicoKine?
Verified customer
Asked: 2019-11-21
Answer
Our laboratory technicains obtained very low sample values by working on normal mouse sera samples which contains little BMP-6. The kit will work for some pathological sera samples in which the expression level of BMP-6 is high.
Boster Scientific Support
Answered: 2019-11-26
Question
Q: if the enzyme conjugated BMP-6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-6 antigen?
Verified Customer
Verified customer
Asked: 2019-02-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-23
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-02-01
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2017-09-29
