Mouse C1R ELISA Kit PicoKine™

Mouse C1R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse C1ra in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1955
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

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Product Name

Mouse C1R ELISA Kit PicoKine™

See all Complement Component C1ra products

SKU/Catalog Number

EK1955

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse C1R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse C1ra in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse C1R ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1955)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Sensitivity

<0.1 ng/ml

Assay Range

0.625 ng/ml - 40 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1955 is reactive to C1ra in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK1955 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1955-CAP Anti-Mouse C1ra Pre-coated 96-well strip microplate 1
EK1955-ST Mouse C1ra Standard 2 vials, 40 ng/tube
EK1955-DA Mouse C1ra Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0480.0910.1790.2590.4550.8181.3211.996

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Complement Component C1ra, Dilution ratio of 1:10000, concentration in serum is around 100ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse C1R ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10043663234061174427224979
Standard deviation47.19197.801451.1779.83196.511448.78
CV (%)4.7%5.4%6.2%6.8%4.6%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Component C1ra in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11004119010381174110181.587.4%
Sample 236634171359936053759238.896.3%
Sample 323406243152039923979230241550.426.7%
*number of samples for each test n=16.

Gene/Protein Information For C1ra (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8CG16

Gene ID

50909

Gene Name

C1ra

Full Name

Complement C1r-A subcomponent

Weight

80.073kDa

Superfamily

peptidase S1 family

Alternative Names

C1ra; Complement Component C1ra C1ra|AI132558, C1r, mC1, mC1rA|complement component 1, r subcomponent A|complement C1r-A subcomponent|complement component 1 subcomponent r-A|complement component 1, r-A subcomponent

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on C1ra, check out the C1ra Infographic

C1ra infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for C1ra: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse C1R ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

R. Varadkar

Verified customer

Asked: 2020-06-24

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-06-24

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

B. Robinson

Verified customer

Asked: 2020-03-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-30

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

G. Parker

Verified customer

Asked: 2019-10-26

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-10-26

Question

Q: What is the optimal O.D. value for Complement Component C1ra ELISA kit? I performed your Complement Component C1ra ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Complement Component C1ra even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Complement Component C1ra ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of Complement Component C1ra that can be declared/interpreted as positive by the above standard is 0.1ng/ml.

Boster Scientific Support

Answered: 2019-07-12

Question

Q: if the enzyme conjugated Complement Component C1ra antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Complement Component C1ra antigen?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-29

Question

Q: can I use heparin plasma as samples in Mouse Complement Component C1ra Picokine® ELISA Kit (Catalog # EK1955)?

Verified Customer

Verified customer

Asked: 2017-11-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Complement Component C1ra causing degradation of its protein structure. Complement Component C1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Complement Component C1ra ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-11-28

Question

Q: how to analyze ELISA data? I have obtained Complement Component C1ra level in urine.

K. Jackson

Verified customer

Asked: 2016-04-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-04-06

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