Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

Carbonic Anhydrase XII/CA12 ELISA kit for Mouse

Mouse CA12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ca12 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1833
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

View all Carbonic Anhydrase XII/CA12 ELISA kits

SKU/Catalog Number

EK1833

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CA12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ca12 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1833)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A25-S301

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1833 is reactive to Ca12 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK1833 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Carbonic Anhydrase XII/CA12

Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 9; 9 C. This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK1833-CAP Anti-Mouse Ca12 Pre-coated 96-well strip microplate 1
EK1833-ST Mouse Ca12 Standard 2 vials, 10 ng/tube
EK1833-DA Mouse Ca12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0100.0310.0640.1370.3760.9221.8792.599

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2410532128111358
Standard deviation1.085.9914.771.436.5526.49
CV (%)4.5%5.7%4.6%5.1%5.9%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase XII/CA12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124242524240.431.7%
Sample 2105921021021004.914.9%
Sample 332127528128829117.786.1%
*number of samples for each test n=16.

Gene/Protein Information For Ca12 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8CI85

Gene ID

Gene Name

Ca12

Full Name

Carbonic anhydrase 12

Weight

39.695kDa

Superfamily

alpha-carbonic anhydrase family

Alternative Names

CA12; Carbonate dehydratase XII; carbonic anhydrase 12; Carbonic Anhydrase XII; carbonic anhydrase XIICAXII; carbonic dehydratase; CA-XII; EC 4.2.1.1; FLJ20151; HsT18816; Tumor antigen HOM-RCC-3.1.3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ca12, check out the Ca12 Infographic

Ca12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ca12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-04-01

Question

Q: how to proceed with the analysis of ELISA data? I have obtained CA12 level in serum.

W. Anand

Verified customer

Asked: 2020-02-28

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-28

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-12-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-12-22

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