Mouse Cathepsin E ELISA Kit PicoKine®

Cathepsin E ELISA kit for Mouse

Mouse Cathepsin E ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ctse in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Product Info Summary

SKU: EK2128
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Mouse Cathepsin E ELISA Kit PicoKine®

View all Cathepsin E ELISA kits

SKU/Catalog Number

EK2128

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Cathepsin E ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ctse in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Cathepsin E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2128)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S60-P397

Sensitivity

<20 pg/ml

Assay Range

0.156 ng/ml - 10 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2128 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2128 is reactive to CTSE in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2128 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cathepsin E

Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Kit Components

Catalog Number Description Quantity
EK2128-CAP Anti-Mouse CTSE Pre-coated 96-well strip microplate 1
EK2128-ST Mouse CTSE Standard 2 vials, 10 ng/tube
EK2128-DA Mouse CTSE Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)00.1560.3120.6251.252.5510
O.D.0.0410.0730.0920.1450.3010.5471.1461.854

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Cathepsin E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)17878527841918052845
Standard deviation7.3034.54141.9810.5131.40173.55
CV(%)4.1%4.4%5.1%5.5%3.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cathepsin E in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 117819118816318012.066.7%
Sample 278569681479377237.834.9%
Sample 327842863292426522806154.325.5%
*number of samples for each test n=16.

Gene/Protein Information For CTSE (Source: Uniprot.Org, NCBI)

Gene Name

CTSE

Full Name

Cathepsin E

Weight

42.794kDa

Superfamily

peptidase A1 family

Alternative Names

CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase CTSE CATE cathepsin E cathepsin E|erythrocyte membrane aspartic proteinase|slow-moving proteinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTSE, check out the CTSE Infographic

CTSE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTSE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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