Mouse CCL22/MDC ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MDC. 96wells/kit, with removable strips. Cited in 2 publication(s).
Mouse CCL22/MDC ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse CCL22/MDC ELISA Kit PicoKine™
See all CCL22/MDC primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse CCL22 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CCL22 tissue specificity: Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.|
|Cite This Product||Mouse CCL22/MDC ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0447)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: G25-S92|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Ccl22 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||20299|
|Species Of This Entry||Mouse|
|Protein Name||C-C motif chemokine 22|
|Superfamily||intercrine beta (chemokine CC) family|
|Alternative Names||CCL22/MDC|A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Oxidation; Acetylation; Nitration; Biotinylation; Methylation; Prenylation|
|Gene Location||8|8 C5, on Chromasome 8, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ccl22 (Source: Uniprot.org, NCBI)
|Protein Function||Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B- lymphocytes with T-cells in immune responses.|
|Tissue Specificity||Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.|
|Related Diseases||Inflammation; Cholangiocarcinoma; Neoplasms; Asthma; Dermatitis; Dermatitis, Atopic; Malignant Neoplasms; Carcinoma; Autoimmune Reaction; Dermatologic Disorders; Tissue Adhesions; Lymphoma; Allergy; Pneumonia; Eosinophilia; Hiv Infections; Malignant Paraganglionic Neoplasm; Leukemia; Inflammatory Response; Decreased Immunologic Activity [pe]|
|Related Pathways||Pathogenesis; Secretion; Immune Response; Chemotaxis; Chemokine Production; Sensitization; Cell Migration; Inflammatory Response; Cell Proliferation; Localization; Cell Activation; T Cell Proliferation; Cytokine Production; Hypersensitivity; T Cell Activation; Cytokine Secretion; Cell Differentiation; Platelet Aggregation; Phagocytosis; Platelet Activation|
|Background||Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.|
|Scientific References||PMID: 9687523 by Schaniel C., et al. Activated murine B lymphocytes and dendritic cells produce a novel CC chemokine which acts selectively on activated T cells.|
Kit Components And Assay QC Data Details Of Mouse CCL22/MDC ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0447-CAP||96-well plate precoated with anti-Mouse CCL22/MDC antibody||1|
|EK0447-ST||lyophilized recombinant Mouse CCL22/MDC standard||10ng/tube|
|EK0447-DA||biotinylated anti-Mouse CCL22/MDC antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0447 Mouse CCL22/MDC ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse CCL22/MDC ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CCL22/MDC ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...||PubMed ID 29247698|
|Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions||PubMed ID 26850448|
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