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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse CCL22/MDC ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse CCL22/MDC ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CCL22/MDC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0447)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: G25-S92
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0447 is reactive to Ccl22 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0447 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL22/MDC
Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
|EK0447-CAP||Anti-Mouse Ccl22 Pre-coated 96-well strip microplate||1|
|EK0447-ST||Mouse Ccl22 Standard||2 vials, 10 ng/tube|
|EK0447-DA||Mouse Ccl22 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CCL22/MDC PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CCL22/MDC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CCL22/MDC in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ccl22 (Source: Uniprot.Org, NCBI)
C-C motif chemokine 22
intercrine beta (chemokine CC) family
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1 Ccl22|ABCD-, ABCD-1, DCBCK, MDC, Scya, Scya22|chemokine (C-C motif) ligand 22|C-C motif chemokine 22|CC chemokine ABCD-1|SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED)|activated B and dendritic cell-derived|dendritic cell and B cell derived chemokine|small inducible cytokine subfamily A, member 22|small inducible cytokine subfamily A22|small-inducible cytokine A22*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ccl22, check out the Ccl22 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0447 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell–cell interactions
Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma
Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha
Activation of RAW264.7 macrophages by active fraction of Albizia julibrissin saponin via Ca2+–ERK1/2–CREB–lncRNA pathways
Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus
He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
EK0447 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Cao, Y., Cao, W., Qiu, Y., Zhou, Y., Guo, Q., Gao, Y., & Lu, N. (2020). Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis. Pharmacological Research, 104981.
Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions
Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...
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