Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0449
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™

See all Ccl3 products

SKU/Catalog Number

EK0449

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0449)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Sensitivity

<10 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0449 is reactive to Ccl3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0449 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0449-CAP Anti-Mouse Ccl3 Pre-coated 96-well strip microplate 1
EK0449-ST Mouse Ccl3 Standard 2 vials, 10 ng/tube
EK0449-DA Mouse Ccl3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.1060.1340.1660.2260.3450.5291.0281.704

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Ccl3, Dilution ratio of 1:1, concentration in serum is around 50pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18742401869249
Standard deviation0.733.999.60.754.9614.44
CV (%)4.1%5.4%4%4.2%7.2%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ccl3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 118191717170.824.8%
Sample 274697669723.084.2%
Sample 324022522820722511.815.2%
*number of samples for each test n=16.

Gene/Protein Information For Ccl3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10855

Gene ID

20302

Gene Name

Ccl3

Full Name

C-C motif chemokine 3

Weight

10.345kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta Ccl3|AI323804, CCL, G0S19-, G0S19-1, LD78, LD78alpha, MIP-, MIP-1alpha, MIP1, MIP1-, MIP1-(a), MIP1-alpha, Mi, Mip1a, Scy, Scya3|chemokine (C-C motif) ligand 3|C-C motif chemokine 3|L2G25B|MIP-1-alpha|MIP1 (a)|SIS-alpha|TY-5|heparin-binding chemotaxis protein|macrophage inflammatory protein 1-alpha|macrophage inflammatory protein-1alpha|small-inducible cytokine A3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl3, check out the Ccl3 Infographic

Ccl3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0449 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The upregulation of integrin ?D?2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis

Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...

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7 Customer Q&As for Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CCL3 using the EK0449 Mouse CCL3 Picokine® ELISA Kit?

L. Miller

Verified customer

Asked: 2019-03-22

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-03-22

Question

Q: which procedure should I follow in order to thaw whole blood sample for CCL3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-08-29

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-08-29

Question

Q: how to analyze ELISA data? I measured CCL3 level in plasma.

Verified Customer

Verified customer

Asked: 2018-08-26

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-26

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CCL3 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

T. Huang

Verified customer

Asked: 2018-07-02

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-07-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Walker

Verified customer

Asked: 2018-01-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-01-18

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-12-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-12-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-06-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-06-03

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