Mouse CD200 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD200. 96wells/kit, with removable strips. Independently reviewed in 1 review(s).
Mouse CD200 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse CD200 ELISA Kit PicoKine™
See all CD200/OX2 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse CD200 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CD200 tissue specificity:|
|Cite This Product||Mouse CD200 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1185)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: Q31-G232|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Cd200 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||17470|
|Species Of This Entry||Mouse|
|Protein Name||OX-2 membrane glycoprotein|
|Alternative Names||CD200/OX2|antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Cleavage|
|Gene Location||16 B5|16 29.53 cM, on Chromasome 16, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cd200 (Source: Uniprot.org, NCBI)
|Protein Function||Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity). .|
|Related Diseases||Inflammation; Neoplasms; Autoimmune Reaction; Malignant Neoplasms; Leukemia; Chronic Lymphocytic Leukemia; Decreased Immunologic Activity [pe]; Nervousness; Lymphoid Leukemia; Autoimmune Diseases; Autoimmunity; Arthritis; Lymphoma; Tissue Adhesions; Influenza; Sclerosis; Neoplasm Metastasis; Experimental Autoimmune Encephalomyelitis; Inflammatory Response|
|Related Pathways||Cytokine Production; Immune Response; Cell Activation; Macrophage Activation; Pathogenesis; Aging; Inflammatory Response; Innate Immune Response; Localization; Secretion; Phagocytosis; Cell Adhesion; T Cell Activation; Cell Proliferation; Cell Migration; Regeneration; Tissue Homeostasis; Cytokine Secretion; Mast Cell Degranulation; Mast Cell Activation|
|Background||OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.|
|Scientific References||PMID: 9434094 by Chen Z., et al. Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene.|
PMID: 9457671 by Borriello F., et al. Characterization and localization of Mox2, the gene encoding the murine homolog of the rat MRC OX-2 membrane glycoprotein.
Kit Components And Assay QC Data Details Of Mouse CD200 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1185-CAP||96-well plate precoated with anti-Mouse CD200/OX2 antibody||1|
|EK1185-ST||lyophilized recombinant Mouse CD200/OX2 standard||10ng/tube|
|EK1185-DA||biotinylated anti-Mouse CD200/OX2 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1185 Mouse CD200 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse CD200 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CD200 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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