Overview
| Product Name |
Mouse CD30L ELISA Kit PicoKine™
See more CD30 Ligand/TNFSF8 products |
| Catalog# |
EK0572 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse CD30L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 6pg/ml. |
| Cite This Product |
Mouse CD30L ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0572)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<6 pg/ml |
| Assay Range |
15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: Q68-D239 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Tnfsf8 (Source: Uniprot.org, NCBI)
| Uniprot Id | P32972 |
|---|
| NCBI Gene Id | 21949 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Tnfsf8 |
|---|
| Protein Name | Tumor necrosis factor ligand superfamily member 8 |
|---|
| Superfamily | tumor necrosis factor family |
|---|
| Alternative Names | CD30 Ligand/TNFSF8|CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8 |
|---|
| Molecular Weight | 26519 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfsf8, check out the Tnfsf8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Tnfsf8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Tnfsf8 infographic
Kit Components And Assay QC Data Details Of Mouse CD30L ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0572-CAP |
Anti-Mouse Tnfsf8 Pre-coated 96-well strip microplate | 1 |
| EK0572-ST | Mouse Tnfsf8 Standard | 2 vials, 10 ng/tube |
| EK0572-DA | Mouse Tnfsf8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0572 Mouse CD30L ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.112 | 0.112 | 0.133 | 0.203 | 0.326 | 0.594 | 1.146 | 2.091 |
We measured random samples of Mouse CD30L ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 254 | 445 | 38 | 267 | 477 |
| Standard deviation | 2.24 | 16.51 | 34.26 | 2.58 | 21.09 | 43.4 |
| CV (%) | 6.4% | 6.5% | 7.7% | 6.8% | 7.9% | 9.1% |
We measured the performance consistency of Mouse CD30L ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 35 | 30 | 34 | 31 | 32 | 2.06 | 6.4% |
| Sample 2 | 254 | 260 | 260 | 224 | 249 | 14.92 | 5.9% |
| Sample 3 | 445 | 507 | 470 | 490 | 478 | 23.11 | 4.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 0
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0572 Mouse CD30L ELISA Kit PicoKine™
5 Related Questions
Question
Q: Can CD30 Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Patel
Asked: 2020-12-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-14
Question
Q: is it okay to use citrate plasma as samples in Mouse CD30 Ligand Picokine® ELISA Kit (Catalog # EK0572)?
Verified Customer
Asked: 2020-08-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD30 Ligand causing disruption of its protein structure. CD30 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD30 Ligand ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-22
Question
Q: how to analyze ELISA data? I have obtained CD30 Ligand level in cell lysates.
Verified Customer
Asked: 2018-10-07
Boster Scientific Support
Answered: 2018-10-07
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD30 Ligand using the EK0572 Mouse CD30 Ligand Picokine® ELISA Kit?
Verified Customer
Asked: 2018-08-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-08-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2016-04-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-04-02
