Mouse CD30L ELISA Kit PicoKine™

Mouse CD30L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml.

Product Info Summary

SKU: EK0572
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD30L ELISA Kit PicoKine™

See all CD30 Ligand/TNFSF8 products

SKU/Catalog Number

EK0572

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD30L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD30L ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0572)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q68-D239

Sensitivity

<6 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0572 is reactive to Tnfsf8 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0572 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0572-CAP Anti-Mouse Tnfsf8 Pre-coated 96-well strip microplate 1
EK0572-ST Mouse Tnfsf8 Standard 2 vials, 10 ng/tube
EK0572-DA Mouse Tnfsf8 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.1120.1120.1330.2030.3260.5941.1462.091

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD30L ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3525444538267477
Standard deviation2.2416.5134.262.5821.0943.4
CV (%)6.4%6.5%7.7%6.8%7.9%9.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD30 Ligand/TNFSF8 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135303431322.066.4%
Sample 225426026022424914.925.9%
Sample 344550747049047823.114.8%
*number of samples for each test n=16.

Gene/Protein Information For Tnfsf8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P32972

Gene ID

21949

Gene Name

Tnfsf8

Full Name

Tumor necrosis factor ligand superfamily member 8

Weight

26.519kDa

Superfamily

tumor necrosis factor family

Alternative Names

CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8 Tnfsf8|CD153, CD30LG, Cd30, Cd30l, Tnlg3a|tumor necrosis factor (ligand) superfamily, member 8|tumor necrosis factor ligand superfamily member 8|CD30 ligand|CD30-L|tumor necrosis factor ligand 3a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfsf8, check out the Tnfsf8 Infographic

Tnfsf8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfsf8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Mouse CD30L ELISA Kit PicoKine™

Question

Q: Can CD30 Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

D. Patel

Verified customer

Asked: 2020-12-14

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-14

Question

Q: is it okay to use citrate plasma as samples in Mouse CD30 Ligand Picokine® ELISA Kit (Catalog # EK0572)?

Verified Customer

Verified customer

Asked: 2020-08-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD30 Ligand causing disruption of its protein structure. CD30 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD30 Ligand ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-22

Question

Q: how to analyze ELISA data? I have obtained CD30 Ligand level in cell lysates.

Verified Customer

Verified customer

Asked: 2018-10-07

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-07

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD30 Ligand using the EK0572 Mouse CD30 Ligand Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-08-07

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-08-07

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-04-02

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-04-02

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