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Product Info Summary
| SKU: | EK0572 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CD30L ELISA Kit PicoKine®
View all CD30 Ligand/TNFSF8 ELISA kits
SKU/Catalog Number
EK0572
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD30L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD30L ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0572)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q68-D239
Sensitivity
<6 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0572 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0572 is reactive to TNFSF8 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0572 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD30 Ligand/TNFSF8
The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0572-CAP | Anti-Mouse TNFSF8 Pre-coated 96-well strip microplate | 1 |
| EK0572-ST | Mouse TNFSF8 Standard | 2 vials, 10 ng/tube |
| EK0572-DA | Mouse TNFSF8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.112 | 0.112 | 0.133 | 0.203 | 0.326 | 0.594 | 1.146 | 2.091 |
Data Example Images
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Mouse CD30L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD30 Ligand/TNFSF8, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD30L ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 254 | 445 | 38 | 267 | 477 |
| Standard deviation | 2.24 | 16.51 | 34.26 | 2.58 | 21.09 | 43.4 |
| CV (%) | 6.4% | 6.5% | 7.7% | 6.8% | 7.9% | 9.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD30 Ligand/TNFSF8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 30 | 34 | 31 | 32 | 2.06 | 6.4% |
| Sample 2 | 254 | 260 | 260 | 224 | 249 | 14.92 | 5.9% |
| Sample 3 | 445 | 507 | 470 | 490 | 478 | 23.11 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For TNFSF8 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF8
Full Name
Tumor necrosis factor ligand superfamily member 8
Weight
26519 MW
Superfamily
tumor necrosis factor family
Alternative Names
CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8 TNFSF8 CD153, CD30L, CD30LG, TNLG3A TNF superfamily member 8 tumor necrosis factor ligand superfamily member 8|CD153 antigen|CD30 antigen ligand|CD30 ligand|CD30-L|tumor necrosis factor (ligand) superfamily, member 8|tumor necrosis factor ligand 3A|tumor necrosis factor superfamily member 8
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF8, check out the TNFSF8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD30L ELISA Kit PicoKine® (EK0572)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Mouse CD30L ELISA Kit PicoKine®
Question
Q: Can CD30 Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Patel
Verified customer
Asked: 2020-12-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-14
Question
Q: is it okay to use citrate plasma as samples in Mouse CD30 Ligand Picokine® ELISA Kit (Catalog # EK0572)?
Verified Customer
Verified customer
Asked: 2020-08-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD30 Ligand causing disruption of its protein structure. CD30 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD30 Ligand ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-22
Question
Q: how to analyze ELISA data? I have obtained CD30 Ligand level in cell lysates.
Verified Customer
Verified customer
Asked: 2018-10-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-07
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD30 Ligand using the EK0572 Mouse CD30 Ligand Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-08-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-04-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-04-02
