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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Chil3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2010)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y22-Y398
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2010 is reactive to Chil3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK2010 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK2010-CAP||Anti-Mouse Chil3 Pre-coated 96-well strip microplate||1|
|EK2010-ST||Mouse Chil3 Standard||2 vials, 10 ng/tube|
|EK2010-DA||Mouse Chil3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CHI3L3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect YM1/Chitinase 3-like 3, Dilution ratio of 1:20, concentration in serum is around 50ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of YM1/Chitinase 3-like 3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Chil3 (Source: Uniprot.Org, NCBI)
Chitinase-like protein 3
glycosyl hydrolase 18 family
AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1 Chil3|AI505981, Chi3, Chi3l3, ECF-L, Ym1|chitinase-like 3|chitinase-like protein 3|beta-N-acetylhexosaminidase Ym1|eosinophil chemotactic cytokine|eosinophil chemotactic factor-L|secreted protein Ym1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Chil3, check out the Chil3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Chil3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK2010
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