Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Chil3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2010
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™

See all YM1/Chitinase 3-like 3 products

SKU/Catalog Number

EK2010

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Chil3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2010)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-Y398

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2010 is reactive to Chil3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2010 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2010-CAP Anti-Mouse Chil3 Pre-coated 96-well strip microplate 1
EK2010-ST Mouse Chil3 Standard 2 vials, 10 ng/tube
EK2010-DA Mouse Chil3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0300.0760.1230.2210.4030.7541.3132.035

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect YM1/Chitinase 3-like 3, Dilution ratio of 1:20, concentration in serum is around 50ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3271000403230810733654
Standard deviation13.7367221.7618.7883.69204.62
CV (%)4.2%6.7%5.5%6.1%7.8%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of YM1/Chitinase 3-like 3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 132733229231931715.434.8%
Sample 21000113411461166111165.385.8%
Sample 340323785449542794147265.896.4%
*number of samples for each test n=16.

Gene/Protein Information For Chil3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O35744

Gene ID

12655

Gene Name

Chil3

Full Name

Chitinase-like protein 3

Weight

44.458kDa

Superfamily

glycosyl hydrolase 18 family

Alternative Names

AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1 Chil3|AI505981, Chi3, Chi3l3, ECF-L, Ym1|chitinase-like 3|chitinase-like protein 3|beta-N-acetylhexosaminidase Ym1|eosinophil chemotactic cytokine|eosinophil chemotactic factor-L|secreted protein Ym1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Chil3, check out the Chil3 Infographic

Chil3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Chil3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK2010

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6 Customer Q&As for Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-01-12

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-01-12

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Y. Thompson

Verified customer

Asked: 2019-09-15

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-15

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-08

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-10-08

Question

Q: is it okay to use EDTA plasma as samples in Mouse YM1 Picokine® ELISA Kit (Catalog # EK2010)?

M. Martinez

Verified customer

Asked: 2018-08-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of YM1 causing degradation of its protein structure. YM1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the YM1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-15

Question

Q: how do I analyze ELISA data? I have obtained YM1 level in serum.

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-15

Question

Q: can you recommend the dilution ratio of serum samples for detection of YM1 in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-05-11

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the YM1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-11

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