Mouse Contactin-1 ELISA Kit PicoKine™

Mouse Contactin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cntn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1772
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Mouse Contactin-1 ELISA Kit PicoKine™

See all Contactin-1 products

SKU/Catalog Number

EK1772

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Contactin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cntn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Contactin-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1772)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D21-S1001

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1772 is reactive to Cntn1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1772 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1772-CAP Anti-Mouse Cntn1 Pre-coated 96-well strip microplate 1
EK1772-ST Mouse Cntn1 Standard 2 vials, 10 ng/tube
EK1772-DA Mouse Cntn1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0070.2250.3360.6961.1191.9112.2202.395

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Contactin-1, Dilution ratio of 1:10, concentration in serum is 20-30ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Contactin-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2981189468430311944854
Standard deviation12.2261.83257.6219.0988.34378.6
CV (%)4.1%5.2%5.5%6.3%7.4%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Contactin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129828832329130013.764.5%
Sample 21189127711391303122766.075.3%
Sample 346844230496448854690284.926.0%
*number of samples for each test n=16.

Gene/Protein Information For Cntn1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P12960

Gene ID

12805

Gene Name

Cntn1

Full Name

Contactin-1

Weight

113.388kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3 Cntn1|AW495098, CN, CNTN, F3ca, F3cam, u, usl|contactin 1|contactin-1|neural cell surface protein F3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cntn1, check out the Cntn1 Infographic

Cntn1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cntn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Mouse Contactin-1 ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for Contactin-1 ELISA after freezing?

L. Gandhi

Verified customer

Asked: 2020-11-30

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Contactin-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-11-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-11

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-17

Question

Q: if the enzyme conjugated Contactin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Contactin-1 antigen?

Z. Krishna

Verified customer

Asked: 2018-05-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-10

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-11-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-11-18

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