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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma ( heparin).|
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Mouse CXCL10/IP-10 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse CXCL10/IP-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl10 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL10/IP-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0736)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: I22-P98
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0736 is reactive to Cxcl10 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin).
EK0736 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL10/IP-10/CRG-2
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
|EK0736-CAP||Anti-Mouse Cxcl10 Pre-coated 96-well strip microplate||1|
|EK0736-ST||Mouse Cxcl10 Standard||2 vials, 10 ng/tube|
|EK0736-DA||Mouse Cxcl10 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CXCL10/IP-10 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL10/IP-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CXCL10/IP-10/CRG-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cxcl10 (Source: Uniprot.Org, NCBI)
C-X-C motif chemokine 10
intercrine alpha (chemokine CxC) family
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 Cxcl10|C7, CRG-, CRG-2, IN, INP10, IP, IP-, IP-10, IP10, Ifi, Ifi10, Scyb1, Scyb10, gIP-, gIP-10, mob-, mob-1|chemokine (C-X-C motif) ligand 10|C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma-IP10|interferon activated gene 10|interferon-gamma induced protein CRG-2|small inducible cytokine B subfamily (Cys-X-Cys), member 10|small-inducible cytokine B10*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cxcl10, check out the Cxcl10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0736 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Y,Wang R,Shi W,Zhihui Z,Wang X,Li C,Zhang S,Zhang P. Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.J Ethnopharmacol.2021 Apr 1:114063.doi:10. 1016/j.jep.2021.114063.Epub ahead of print.PMID:33813013.
EK0736 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination
The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors
ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
USP44 positively regulates innate immune response to DNA viruses through deubiquitinating MITA
Activation of CXCL10/CXCR3 signaling attenuates morphine analgesia: involvement of Gi protein
Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
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