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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma ( heparin, EDTA) and urine.|
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Mouse Cystatin C/CST3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse Cystatin C/CST3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cst3 in cell culture supernatants, serum, plasma ( heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse Cystatin C/CST3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0679)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-A140
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0679 is reactive to Cst3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma ( heparin, EDTA) and urine.
EK0679 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0679-CAP||Anti-Mouse Cst3 Pre-coated 96-well strip microplate||1|
|EK0679-ST||Mouse Cst3 Standard||2 vials, 20 ng/tube|
|EK0679-DA||Mouse Cst3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse Cystatin C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cystatin C, Dilution ratio of 1:200, concentration in serum is around 800ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Cystatin C/CST3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cst3 (Source: Uniprot.Org, NCBI)
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin Cst3|Cys, CysC|cystatin C|cystatin-C|cystatin-3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cst3, check out the Cst3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cst3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0679 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
EK0679 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Activated iRhom2 drives prolonged PM2.5 exposure-triggered renal injury in Nrf2-defective mice
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