Mouse DLL4 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DLL4. 96wells/kit, with removable strips.
Mouse DLL4 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse DLL4 ELISA Kit PicoKine™
See all Dll4 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse DLL4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. DLL4 tissue specificity: Expressed in vascular endothelium. Expressed in retina at least during embryogenesis. .|
|Cite This Product||Mouse DLL4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1277)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: S28-P525|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Dll4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||54485|
|Species Of This Entry||Mouse|
|Protein Name||Delta-like protein 4|
|Alternative Names||Dll4|Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Acetylation; Deacetylation; Methylation; Ubiquitination|
|Gene Location||2|2 E5, on Chromasome 2, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Dll4 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (By similarity). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (PubMed:22323600). During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity).|
|Tissue Specificity||Expressed in vascular endothelium. Expressed in retina at least during embryogenesis.|
|Related Diseases||Tumor Angiogenesis; Neoplasms; Pathologic Neovascularization; Malignant Neoplasms; Hypoxia; Carcinoma; Leukemia; Neoplasm Metastasis; Malignant Neoplasm Of Breast; Mammary Neoplasms; Malignant Paraganglionic Neoplasm; Cell Invasion; Inflammation; Tissue Adhesions; Cell Transformation, Neoplastic; Lung Neoplasms; Sarcoma; Adenocarcinoma|
|Related Pathways||Angiogenesis; Cell Proliferation; Notch Signaling Pathway; Cell Differentiation; Sprouting Angiogenesis; Pathogenesis; Cell Development; Cell Cycle; Cell Growth; Localization; Endothelial Cell Proliferation; Cell Migration; Vasculogenesis; Tube Formation; Neurogenesis; Cell Fate Determination; Cell Activation; Lateral Inhibition; Regeneration; T Cell Differentiation|
|Background||Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.|
|Scientific References||PMID: 10837024 by Shutter J.R., et al. Dll4, a novel Notch ligand expressed in arterial endothelium.|
PMID: 11134954 by Yoneya T., et al. Molecular cloning of Delta-4, a new mouse and human Notch ligand.
Kit Components And Assay QC Data Details Of Mouse DLL4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1277-CAP||96-well plate precoated with anti-Mouse Dll4 antibody||1|
|EK1277-ST||lyophilized recombinant Mouse Dll4 standard||10ng/tube|
|EK1277-DA||biotinylated anti-Mouse Dll4 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1277 Mouse DLL4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse DLL4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse DLL4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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