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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Mouse ENTPD2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse ENTPD2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Entpd2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ENTPD2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2014)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: C26-S462
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2014 is reactive to Entpd2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK2014 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD39L1/ENTPD2
Ectonucleoside triphosphate diphosphohydrolase 2 is an enzyme that in humans is encoded by the ENTPD2 gene. This gene is mapped to 2; 2 A3. The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.
|EK2014-CAP||Anti-Mouse Entpd2 Pre-coated 96-well strip microplate||1|
|EK2014-ST||Mouse Entpd2 Standard||2 vials, 10 ng/tube|
|EK2014-DA||Mouse Entpd2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse ENTPD2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD39L1/ENTPD2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ENTPD2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD39L1/ENTPD2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Entpd2 (Source: Uniprot.Org, NCBI)
Ectonucleoside triphosphate diphosphohydrolase 2
GDA1/CD39 NTPase family
CD39 antigen-like 1; CD39L1; CD39L1NTPDase 2; CD39-like-1; EC 3.6.1; EC 3.6.1.-; EC 126.96.36.199; Ecto-ATP diphosphohydrolase 2; Ecto-ATPase 2; Ecto-ATPase; Ecto-ATPDase 2; ectonucleoside triphosphate diphosphohydrolase 2; ENTPD2; NTPD2; NTPDase 2; NTPDase-2 Entpd2|Cd39l, Cd39l1, NTPD, NTPDase2|ectonucleoside triphosphate diphosphohydrolase 2|ectonucleoside triphosphate diphosphohydrolase 2|CD39 antigen-like 1|NTPDase 2|ecto-ATP diphosphohydrolase 2|ecto-ATPDase 2|ecto-ATPase 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Entpd2, check out the Entpd2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Entpd2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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