Click image to see more details
Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
Customers Who Bought This Also Bought
Mouse FAS/Cd95 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse FAS/Cd95 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fas in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FAS/Cd95 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0336)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q22-R169
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0336 is reactive to Fas in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0336 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FAS
Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.
|EK0336-CAP||Anti-Mouse Fas Pre-coated 96-well strip microplate||1|
|EK0336-ST||Mouse Fas Standard||2 vials, 10 ng/tube|
|EK0336-DA||Mouse Fas Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Mouse FAS PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FAS/Cd95 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FAS in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Fas (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 6
ALPS1A; APO-1 cell surface antigen; Apo-1; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; APT1; CD95 antigen; CD95; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6 FAS ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS Fas cell surface death receptor tumor necrosis factor receptor superfamily member 6|APO-1 cell surface antigen|CD95 antigen|FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas AMA|TNF receptor superfamily member 6|apoptosis antigen 1|apoptosis signaling receptor FAS|apoptosis-mediating surface antigen FAS|mutant tumor necrosis receptor superfamily member 6|tumor necrosis factor receptor superfamily, member 6*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Fas, check out the Fas Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fas: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0336 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Potential Analysis and Preparation of Chitosan Oligosaccharides as Oral Nutritional Supplements of Cancer Adjuvant Therapy
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Mouse FAS/Cd95 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Mouse FAS/Cd95 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question