Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine™

Mouse Fc gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fcgr2b/Fcgr3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1313
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine™

See all Fcgr2b/Fcgr3 products

SKU/Catalog Number

EK1313

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Fc gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fcgr2b/Fcgr3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1313)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T40-R217

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1313 is reactive to Fcgr2b/Fcgr3 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1313 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1313-CAP Anti-Mouse Fcgr2b/Fcgr3 Pre-coated 96-well strip microplate 1
EK1313-ST Mouse Fcgr2b/Fcgr3 Standard 2 vials, 10 ng/tube
EK1313-DA Mouse Fcgr2b/Fcgr3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0040.0910.1550.2900.4910.8301.4842.103

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8921693294236885
Standard deviation5.0516.6346.66.9520.0646.9
CV (%)5%7.7%5%7.4%8.5%5.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fcgr2b/Fcgr3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 189879886904.745.2%
Sample 221623522920622111.285.1%
Sample 393293584184888944.585%
*number of samples for each test n=16.

Gene/Protein Information For Fcgr2b/Fcgr3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08101/P08508

Gene ID

Gene Name

Fcgr2b/Fcgr3

Full Name

Weight

36695 MW

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II;Fc gamma receptor IIB;Fc-gamma RII;Fc-gamma-RIIB;FcRII;IgG Fc receptor II beta;Lymphocyte antigen 17;Ly-17;CD32;Fcgr2;Fcgr2b, Ly-17;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fcgr2b/Fcgr3, check out the Fcgr2b/Fcgr3 Infographic

Fcgr2b/Fcgr3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fcgr2b/Fcgr3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1313

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7 Customer Q&As for Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine™

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-09-03

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-03

Question

Q: if the enzyme conjugated Fcgr2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fcgr2 antigen?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-05

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Fcgr2 using the EK1313 Mouse Fcgr2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-11-25

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Fcgr2 in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

S. Singh

Verified customer

Asked: 2019-06-30

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Fcgr2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-30

Question

Q: Can Fcgr2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Harris

Verified customer

Asked: 2018-11-26

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-11-26

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-07-31

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-07-31

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Walker

Verified customer

Asked: 2018-06-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-30

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