Mouse FOLR1/Alpha Fr PicoKine™ ELISA Kit
|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Product Name||Mouse FOLR1/Alpha Fr PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FOLR1. 96wells/kit, with removable strips.|
|Cite This Product||Mouse FOLR1/Alpha Fr PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1243)|
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility.
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: T25-S232|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|EK1243-CAP||96-well plate precoated with anti-Mouse Folr1 antibody||1|
|EK1243-ST||lyophilized recombinant Mouse Folr1 standard||10ng/tube|
|EK1243-DA||biotinylated anti-Mouse Folr1 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Mouse FOLR1/Alpha Fr PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Folate receptor alpha|
|Tissue Specificity||Detected in kidney proximal tubules (at protein level). .|
|Alternative Names||Folate receptor alpha;FR-alpha;Folate receptor 1;Folate-binding protein 1;Folr1;Fbp1, Folbp1;|
|Subcellular Localization||Cell membrane; Lipid-anchor, GPI-anchor. Secreted . Cytoplasmic vesicle . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Apical cell membrane. Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane (By similarity). Detected at proximal tubule apical membranes. .|
|Molecular Weight||29449 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption. .|
|Background||Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.|
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The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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