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Product Info Summary
| SKU: | EK1370 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®
SKU/Catalog Number
EK1370
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Hjv in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1370)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q33-D393
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1370 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1370 is reactive to Hjv in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1370 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Hjv
Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1370-CAP | Anti-Mouse Hjv Pre-coated 96-well strip microplate | 1 |
| EK1370-ST | Mouse Hjv Standard | 2 vials, 10 ng/tube |
| EK1370-DA | Mouse Hjv Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.033 | 0.13 | 0.219 | 0.279 | 0.5 | 0.814 | 1.246 | 1.919 |
Data Example Images
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Mouse Hemojuvelin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Hjv, Dilution ratio of 1:1, concentration in serum and plasma is 600-900 pg/ml..
Some articles we found to cite concentrations of Hjv in samples: 22875629 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 174 | 404 | 1769 | 170 | 398 | 1835 |
| Standard deviation | 11.48 | 18.98 | 714.453 | 14.45 | 25.87 | 114.7% |
| CV (%) | 6.6% | 4.7% | 4.4% | 8.5% | 6.5% | 6.3% |
Specific Publications For Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (EK1370)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Mouse Hfe2 Picokine® ELISA Kit (Catalog # EK1370)?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Hfe2 causing degradation of its protein structure. Hfe2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Hfe2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-07-13
Question
Q: What is the optimal O.D. value for Hfe2 ELISA kit? I used your Hfe2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Hfe2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Hfe2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Hfe2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-05-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
D. Garcia
Verified customer
Asked: 2018-03-13
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-12
Question
Q: how to thaw whole blood sample for Hfe2 ELISA after freezing?
E. Puri
Verified customer
Asked: 2016-11-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Hfe2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-11-03
