Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)

IL-1 alpha/IL-1F1 ELISA antibody pairs, Mouse, for 5 plates

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il1a in cell culture supernatants and serum. Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ0391
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL-1 alpha/IL-1F1 products

SKU/Catalog Number

EZ0391

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il1a in cell culture supernatants and serum. Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0391)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S115-S270

Sensitivity

<1 pg/ml

Assay Range

4.69 pg/ml - 300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0391 is reactive to Il1a in Mouse samples

Validated Sample Types

cell culture supernatants and serum

Application Guarantee

EZ0391 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0391-CA Mouse anti- mouse Il1a monoclonal antibody (Capture Antibody) 500 µl, 4 µg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0391-DA Biotinylated goat anti- mouse Il1a polyclonal antibody (Detection Antibody) 500 µl, 0.15 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0391-ST Lyophilized recombinant mouse Il1a standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04.79.418.837.575150300
O.D.0.0460.1120.1420.2400.4300.7601.2701.871

Data Example Images

Gene/Protein Information For Il1a (Source: Uniprot.Org, NCBI)

Uniprot ID

P01582

Gene ID

16175

Gene Name

Il1a

Full Name

Interleukin-1 alpha

Weight

31.023kDa

Superfamily

IL-1 family

Alternative Names

BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha Il1a|Il, Il-1a|interleukin 1 alpha|interleukin-1 alpha|IL-1 alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1a, check out the Il1a Infographic

Il1a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EZ0391

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)

$255

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
EZ0391
$255.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.