Introduction
ARG1 catalyzes the hydrolysis of arginine to ornithine and urea. 2 isoforms of mammalian arginase exist which vary in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic role. ARG1 is a cytosolic enzyme and expressed widely in the liver as part of the urea cycle.
Inherited deficiency of this ARG1 causes argininemia, which is an autosomal recessive disorder characterized by hyperammonemia.
Overview
| Product Name |
Mouse IL-23 ELISA Kit PicoKine™
See all IL-23A/IL-23 P19 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0866 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Mouse Il 23/IL23A with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 23/IL23A tissue specificity: Secreted by activated dendritic cells (at protein level). Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. . |
| Cite This Product |
Mouse IL-23 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0866)
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum and plasma(heparin). |
| Sensitivity |
<2pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: sf21; Immunogen sequence: (M23-S335)+(L20-A196) |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Il23a (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9EQ14 |
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| NCBI Gene Id | 83430 |
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| Species Of This Entry | Mouse |
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| Gene Name | Il23a |
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| Protein Name | Interleukin-23 subunit alpha |
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| Superfamily | IL-6 superfamily |
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| Alternative Names | IL-23A/IL-23 P19|IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha |
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| Molecular Weight | 22071 |
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| Post Tranlational Modifications | Phosphorylation; Glycosylation; Cleavage; Deacetylation; Oxidation |
|---|
| Gene Location | 10|10 D3, on Chromasome 10, gene sequence: |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il23a (Source: Uniprot.org, NCBI)
| Protein Function | Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
|---|
| Tissue Specificity | Secreted by activated dendritic cells (at protein level). Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. |
|---|
| Related Diseases | Inflammation; Autoimmune Reaction; Autoimmune Diseases; Neoplasms; Autoimmunity; Experimental Autoimmune Encephalomyelitis; Arthritis; Psoriasis; Encephalomyelitis; Infective Disorder; Tuberculosis; Multiple Sclerosis; Salmonella Infections; Sclerosis; Inflammatory Bowel Diseases; Mycobacterium Infections; Crohn Disease; Colitis; Intestinal Diseases; Nervousness |
|---|
| Related Pathways | Immune Response; Pathogenesis; Secretion; Cytokine Production; Cell Activation; Interferon-gamma Production; Inflammatory Response; Cell Differentiation; Hypersensitivity; Reverse Transcription; Translation; Granuloma Formation; Cell Proliferation; Cell Development; Angiogenesis; T Cell Activation; Virulence; Phagocytosis; T Cell Proliferation; Innate Immune Response |
|---|
| Background | Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812). |
|---|
| Scientific References | PMID: 11114383 by Oppmann B., et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
PMID: 11390512 by Wiekowski M.T., et al. Ubiquitous transgenic expression of the IL-23 subunit p19 induces multiorgan inflammation, runting, infertility, and premature death. |
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Kit Components And Assay QC Data Details Of Mouse IL-23 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0866-CAP | 96-well plate precoated with anti-Mouse IL-23A/IL-23 P19 antibody | 1 |
| EK0866-ST | lyophilized recombinant Mouse IL-23A/IL-23 P19 standard | 10ng/tube |
| EK0866-DA | biotinylated anti-Mouse IL-23A/IL-23 P19 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0866 Mouse IL-23 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 |
| O.D. | 0.168 | 0.198 | 0.242 | 0.355 | 0.547 | 0.935 | 1.561 | 2.391 |
We measured random samples of Mouse IL-23 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 93 | 278 | 780 | 85 | 279 | 762 |
| Standard deviation | 4.09 | 13.9 | 34.32 | 4.4%7 | 19.25 | 38.86 |
| CV(%) | 4.4% | 5% | 4.4% | 6.2% | 6.9% | 5.1% |
We measured the performance consistency of Mouse IL-23 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 93 | 84 | 99 | 94 | 92 | 5.4 | 5.8% |
| Sample 2 | 278 | 287 | 274 | 301 | 285 | 10.36 | 3.6% |
| Sample 3 | 780 | 941 | 844 | 875 | 860 | 57.96 | 6.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all IL-23A/IL-23 P19 primary antibodies,
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Total number of citations: 4
| Zhang Hq, Ding Tt, Zhao Js, Yang X, Zhang Hx, Zhang Jj, Cui Yl. World J Gastroenterol. 2009 Apr 21;15(15):1821-8. Therapeutic Effects Of Clostridium Butyricum On Experimental Colitis Induced By Oxazolone In Rats. |
PubMed ID 19370778 |
| MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response |
PubMed ID 27725852 |
| Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia |
PubMed ID 25627661 |
| %u516D%u541B%u5B50%u6C64%u63D0%u53D6%u7269%u6291%u5236%u98DF%u7BA1%u764C Ec9706 %u7EC6%u80DE%u589E%u6B96%u53CA%u5176%u5BF9%u4FE1%u53F7%u8F6C%u5BFC%u548C%u8F6C%u5F55%u6D3B%u5316%u56E0%u5B50 3 %u4FE1%u53F7%u901A%u8DEF%u7684%u5F71%u54CD |
PubMed ID 30362164 |
