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Product Info Summary
| SKU: | EK0866 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
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Product info
Product Name
Mouse IL-23 ELISA Kit PicoKine™
See all IL-23A/IL-23 P19 products
SKU/Catalog Number
EK0866
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-23 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il23a in cell culture supernatants, serum and plasma (heparin). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-23 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0866)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196)
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0866 is reactive to Il23a in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0866 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0866-CAP | Anti-Mouse Il23a Pre-coated 96-well strip microplate | 1 |
| EK0866-ST | Mouse Il23a Standard | 2 vials, 10 ng/tube |
| EK0866-DA | Mouse Il23a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.093 | 0.168 | 0.198 | 0.242 | 0.355 | 0.547 | 0.935 | 1.961 |
Data Example Images
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Mouse IL-23 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-23A/IL-23 P19, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-23 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 93 | 278 | 780 | 85 | 279 | 762 |
| Standard deviation | 4.09 | 13.9 | 34.32 | 4.4%7 | 19.25 | 38.86 |
| CV (%) | 4.4% | 5% | 4.4% | 6.2% | 6.9% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-23A/IL-23 P19 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 93 | 84 | 99 | 94 | 92 | 5.4 | 5.8% |
| Sample 2 | 278 | 287 | 274 | 301 | 285 | 10.36 | 3.6% |
| Sample 3 | 780 | 941 | 844 | 875 | 860 | 57.96 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For Il23a (Source: Uniprot.Org, NCBI)
Uniprot ID
Q9EQ14
Gene ID
83430
Gene Name
Il23a
Full Name
Interleukin-23 subunit alpha
Weight
22.071kDa
Superfamily
IL-6 superfamily
Alternative Names
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha Il23a|IL-, IL-23, p1, p19|interleukin 23, alpha subunit p19|interleukin-23 subunit alpha|IL-23 subunit alpha|IL-23-A|IL-23p19|interleukin-23 subunit p19
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il23a, check out the Il23a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il23a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse IL-23 ELISA Kit PicoKine™ (EK0866)
EK0866 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li B,Du P,Du Y,Zhao D,Cai Y,Yang Q,Guo Z.Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats.Life Sci.2021 Jan 9;269:119008.doi:10.1016/j.lfs.2020.119008.Epub ahead of print.PMID:33434535.
Species: Rat
EK0866 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response
Zhang Hq, Ding Tt, Zhao Js, Yang X, Zhang Hx, Zhang Jj, Cui Yl. World J Gastroenterol. 2009 Apr 21;15(15):1821-8. Therapeutic Effects Of Clostridium Butyricum On Experimental Colitis Induced By Oxazolone In Rats.
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Mouse IL-23 ELISA Kit PicoKine™
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-12-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-12-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-23A using the EK0866 Mouse IL-23A Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-10-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-19
Question
Q: if the enzyme conjugated IL-23A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-23A antigen?
Verified Customer
Verified customer
Asked: 2020-04-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Zhang
Verified customer
Asked: 2019-01-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
L. Evans
Verified customer
Asked: 2018-03-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-16
Question
Q: can I use EDTA plasma as samples in Mouse IL-23A Picokine® ELISA Kit (Catalog # EK0866)?
Y. Puri
Verified customer
Asked: 2016-10-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-23A causing disruption of its protein structure. IL-23A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-23A ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-10-09
Question
Q: how to analyze ELISA data? I have obtained IL-23A level in plasma.
Verified Customer
Verified customer
Asked: 2015-01-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-01-29
Question
Just want to know if the product EK0866 antibody kit can be used to detect in mouse brain tissue homogenate?
Verified Customer
Verified customer
Asked: 2014-07-18
Answer
The product EK0866 antibody kit have been validated and tested only in culture supernatant, serum and citrate plasma, however it might be suitable to use in untested sample types like tissue lysates.
Boster Scientific Support
Answered: 2014-07-18
