Overview
| Product Name |
Mouse IL-23 ELISA Kit PicoKine™
See more IL-23A/IL-23 P19 products |
| Catalog# |
EK0866 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse IL-23 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il23a in cell culture supernatants, serum and plasma (heparin).. Sensitivity: 2pg/ml. |
| Cite This Product |
Mouse IL-23 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0866)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma (heparin). |
| Sensitivity |
<2 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196) |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Il23a (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9EQ14 |
|---|
| NCBI Gene Id | 83430 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Il23a |
|---|
| Protein Name | Interleukin-23 subunit alpha |
|---|
| Superfamily | IL-6 superfamily |
|---|
| Alternative Names | IL-23A/IL-23 P19|IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha |
|---|
| Molecular Weight | 22071 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il23a, check out the Il23a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Il23a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Il23a infographic
Kit Components And Assay QC Data Details Of Mouse IL-23 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0866-CAP |
Anti-Mouse Il23a Pre-coated 96-well strip microplate | 1 |
| EK0866-ST | Mouse Il23a Standard | 2 vials, 10 ng/tube |
| EK0866-DA | Mouse Il23a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0866 Mouse IL-23 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.168 | 0.198 | 0.242 | 0.355 | 0.547 | 0.935 | 1.561 | 2.391 |
We measured random samples of Mouse IL-23 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 93 | 278 | 780 | 85 | 279 | 762 |
| Standard deviation | 4.09 | 13.9 | 34.32 | 4.4%7 | 19.25 | 38.86 |
| CV (%) | 4.4% | 5% | 4.4% | 6.2% | 6.9% | 5.1% |
We measured the performance consistency of Mouse IL-23 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 93 | 84 | 99 | 94 | 92 | 5.4 | 5.8% |
| Sample 2 | 278 | 287 | 274 | 301 | 285 | 10.36 | 3.6% |
| Sample 3 | 780 | 941 | 844 | 875 | 860 | 57.96 | 6.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
Li B,Du P,Du Y,Zhao D,Cai Y,Yang Q,Guo Z.Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats.Life Sci.2021 Jan 9;269:119008.doi:10.1016/j.lfs.2020.119008.Epub ahead of print.PMID:33434535.
Species: Rat
EK0866 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA |
PubMed ID 33434535 |
| MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response |
PubMed ID 27725852 |
| Zhang Hq, Ding Tt, Zhao Js, Yang X, Zhang Hx, Zhang Jj, Cui Yl. World J Gastroenterol. 2009 Apr 21;15(15):1821-8. Therapeutic Effects Of Clostridium Butyricum On Experimental Colitis Induced By Oxazolone In Rats. |
PubMed ID 19370778 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0866 Mouse IL-23 ELISA Kit PicoKine™
9 Related Questions
Question
Just want to know if the product EK0866 antibody kit can be used to detect in mouse brain tissue homogenate?
Verified Customer
Asked: 2014-07-18
Answer
The product EK0866 antibody kit have been validated and tested only in culture supernatant, serum and citrate plasma, however it might be suitable to use in untested sample types like tissue lysates.
Boster Scientific Support
Answered: 2014-07-18
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-12-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-12-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-23A using the EK0866 Mouse IL-23A Picokine® ELISA Kit?
Verified Customer
Asked: 2020-11-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2020-10-19
Boster Scientific Support
Answered: 2020-10-19
Question
Q: if the enzyme conjugated IL-23A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-23A antigen?
Verified Customer
Asked: 2020-04-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Zhang
Asked: 2019-01-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
L. Evans
Asked: 2018-03-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-16
Question
Q: can I use EDTA plasma as samples in Mouse IL-23A Picokine® ELISA Kit (Catalog # EK0866)?
Y. Puri
Asked: 2016-10-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-23A causing disruption of its protein structure. IL-23A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-23A ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-10-09
Question
Q: how to analyze ELISA data? I have obtained IL-23A level in plasma.
Verified Customer
Asked: 2015-01-29
Boster Scientific Support
Answered: 2015-01-29
