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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Mouse IL-23 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IL-23 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il23a in cell culture supernatants, serum and plasma (heparin). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-23 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0866)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196)
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0866 is reactive to Il23a in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0866 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0866-CAP||Anti-Mouse Il23a Pre-coated 96-well strip microplate||1|
|EK0866-ST||Mouse Il23a Standard||2 vials, 10 ng/tube|
|EK0866-DA||Mouse Il23a Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IL-23 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-23A/IL-23 P19, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-23 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-23A/IL-23 P19 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Il23a (Source: Uniprot.Org, NCBI)
Interleukin-23 subunit alpha
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha Il23a|IL-, IL-23, p1, p19|interleukin 23, alpha subunit p19|interleukin-23 subunit alpha|IL-23 subunit alpha|IL-23-A|IL-23p19|interleukin-23 subunit p19*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il23a, check out the Il23a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il23a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0866 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li B,Du P,Du Y,Zhao D,Cai Y,Yang Q,Guo Z.Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats.Life Sci.2021 Jan 9;269:119008.doi:10.1016/j.lfs.2020.119008.Epub ahead of print.PMID:33434535.
EK0866 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response
Zhang Hq, Ding Tt, Zhao Js, Yang X, Zhang Hx, Zhang Jj, Cui Yl. World J Gastroenterol. 2009 Apr 21;15(15):1821-8. Therapeutic Effects Of Clostridium Butyricum On Experimental Colitis Induced By Oxazolone In Rats.
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