Mouse LDLR / OLR1 ELISA Kit PicoKine™

Mouse LDLR / OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ldlr in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 12pg/ml.

Product Info Summary

SKU: EK1543
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and urine.

Product Name

Mouse LDLR / OLR1 ELISA Kit PicoKine™

See all Ldlr products

SKU/Catalog Number

EK1543

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse LDLR / OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ldlr in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 12pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse LDLR / OLR1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1543)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-R790

Sensitivity

<12 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1543 is reactive to Ldlr in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and urine.

Application Guarantee

EK1543 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1543-CAP Anti-Mouse Ldlr Pre-coated 96-well strip microplate 1
EK1543-ST Mouse Ldlr Standard 2 vials, 10 ng/tube
EK1543-DA Mouse Ldlr Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04000200040002000400020004000
O.D.0.0190.1630.2610.4060.6921.0981.6821.981

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Ldlr, Dilution ratio of 1:40, concentration in serum is around 32ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse LDLR / OLR1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13859914721316501349
Standard deviation7.3128.1588.327.3342.9102.52
CV (%)5.3%4.7%6%5.6%6.6%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ldlr in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11381411371311363.632.6%
Sample 259952256452955330.715.5%
Sample 31472133314471397141253.123.7%
*number of samples for each test n=16.

Gene/Protein Information For Ldlr (Source: Uniprot.Org, NCBI)

Uniprot ID

P35951

Gene ID

16835

Gene Name

Ldlr

Full Name

Low-density lipoprotein receptor

Weight

94.947kDa

Superfamily

LDLR family

Alternative Names

FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor Ldlr|Hlb301|low density lipoprotein receptor|low-density lipoprotein receptor|LDL receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ldlr, check out the Ldlr Infographic

Ldlr infographic

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In this infographic you will see the following information for Ldlr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Mouse LDLR / OLR1 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Adams

Verified customer

Asked: 2019-10-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-10-30

Question

Q: which procedure should I follow in order to thaw whole blood sample for LDLR ELISA after freezing?

L. Perez

Verified customer

Asked: 2019-01-12

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LDLR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-01-12

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring LDLR using the EK1543 Mouse LDLR Picokine® ELISA Kit?

R. Thompson

Verified customer

Asked: 2018-08-27

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-08-27

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

K. Edwards

Verified customer

Asked: 2018-01-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-01-04

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of LDLR in Mouse urine? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-03-27

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LDLR ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-03-27

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