Mouse LTBR ELISA Kit PicoKine™

Mouse LTBR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ltbr in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2003
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse LTBR ELISA Kit PicoKine™

See all Lymphotoxin beta R/TNFRSF3 products

SKU/Catalog Number

EK2003

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse LTBR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ltbr in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse LTBR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2003)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q31-L223

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2003 is reactive to Ltbr in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2003 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2003-CAP Anti-Mouse Ltbr Pre-coated 96-well strip microplate 1
EK2003-ST Mouse Ltbr Standard 2 vials, 10 ng/tube
EK2003-DA Mouse Ltbr Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0370.0630.1430.2850.5231.0371.6782.412

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Lymphotoxin beta R/TNFRSF3, Dilution ratio of 1:1, concentration in serum is around 60pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse LTBR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13655224511415822471
Standard deviation7.0729.26134.818.1836.08163.09
CV (%)5.2%5.3%5.5%5.8%6.2%6.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lymphotoxin beta R/TNFRSF3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11361481501271409.336.6%
Sample 255255755259656418.443.2%
Sample 324512253243322192339103.894.4%
*number of samples for each test n=16.

Gene/Protein Information For Ltbr (Source: Uniprot.Org, NCBI)

Uniprot ID

P50284

Gene ID

17000

Gene Name

Ltbr

Full Name

Tumor necrosis factor receptor superfamily member 3

Weight

44.956kDa

Alternative Names

CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III Ltbr|AI256028, L, LTbe, LTbetaR, Ltar, TN, TNF-, TNF-R-III, TNFCR, TNFR, TNFR-RP, TNFR2-RP, TNFRrp, Tnf, Tnfbr, Tnfrsf3|lymphotoxin B receptor|tumor necrosis factor receptor superfamily member 3|LT-beta receptor|TNF receptor-related protein|lymphotoxin-beta receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ltbr, check out the Ltbr Infographic

Ltbr infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ltbr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse LTBR ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Mouse Lymphotoxin beta R Picokine® ELISA Kit (Catalog # EK2003)?

Verified Customer

Verified customer

Asked: 2020-07-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Lymphotoxin beta R causing degradation of its protein structure. Lymphotoxin beta R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Lymphotoxin beta R ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-07-12

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Lymphotoxin beta R level in serum.

K. White

Verified customer

Asked: 2019-12-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-12-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

S. Dayal

Verified customer

Asked: 2019-05-06

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-05-06

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

C. Adams

Verified customer

Asked: 2018-06-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-13

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-05-16

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-16

Question

Q: What is the optimal O.D. value for Lymphotoxin beta R ELISA kit? I used your Lymphotoxin beta R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Lymphotoxin beta R even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-10-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lymphotoxin beta R ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lymphotoxin beta R that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-10-03

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