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Product Info Summary
| SKU: | EK2263 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse MARCO ELISA Kit PicoKine®
SKU/Catalog Number
EK2263
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MARCO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MARCO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MARCO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2263)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q70-S518
Sensitivity
<150 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2263 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2263 is reactive to MARCO in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2263 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MARCO
Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the MARCO gene. The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2263-CAP | Anti-Mouse MARCO Pre-coated 96-well strip microplate | 1 |
| EK2263-ST | Mouse MARCO Standard | 2 vials, 20 ng/tube |
| EK2263-DA | Mouse MARCO Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.016 | 0.045 | 0.089 | 0.178 | 0.323 | 0.767 | 1.251 | 1.873 |
Data Example Images
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Mouse MARCO PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MARCO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 555 | 709 | 7761 | 438 | 1039 | 5548 |
| Standard deviation | 30.53 | 44.88 | 305.01 | 27.81 | 41.04 | 220.81 |
| CV(%) | 5.5% | 6.3% | 3.9% | 6.4% | 4% | 4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MARCO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 555 | 600 | 343 | 464 | 491 | 21.29 | 4.3% |
| Sample 2 | 709 | 1133 | 780 | 1104 | 932 | 47.79 | 5.1% |
| Sample 3 | 7761 | 5570 | 7930 | 9513 | 7694 | 168.49 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For MARCO (Source: Uniprot.Org, NCBI)
Gene Name
MARCO
Full Name
Macrophage receptor MARCO
Weight
52.658kDa
Alternative Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2 MARCO SCARA2, SR-A6 macrophage receptor with collagenous structure macrophage receptor MARCO|scavenger receptor class A, member 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MARCO, check out the MARCO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MARCO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MARCO ELISA Kit PicoKine® (EK2263)
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