Mouse Matrilin-2 ELISA Kit PicoKine®

Matrilin-2 ELISA kit for Mouse

Mouse Matrilin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Matn2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2021
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse Matrilin-2 ELISA Kit PicoKine®

View all Matrilin-2 ELISA kits

SKU/Catalog Number

EK2021

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Matrilin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Matn2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Matrilin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2021)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R24-R956

Sensitivity

<50 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2021 is reactive to Matn2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2021 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Matrilin-2

Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 15; 15 B3.1. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.

Kit Components

Catalog Number Description Quantity
EK2021-CAP Anti-Mouse Matn2 Pre-coated 96-well strip microplate 1
EK2021-ST Mouse Matn2 Standard 2 vials, 10 ng/tube
EK2021-DA Mouse Matn2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.5375750150030006000
O.D.0.0440.1320.2120.3460.5911.0631.6592.189

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Matrilin-2, Dilution ratio of 1:5, concentration in serum is 300-3200pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Matrilin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)17865723961816852417
Standard deviation8.0137.45110.229.2340.42178.86
CV (%)4.5%5.7%4.6%5.1%5.9%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Matrilin-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11781941841851855.713.0%
Sample 265767060059162934.455.4%
Sample 32396228924142332235750.042.1%
*number of samples for each test n=16.

Gene/Protein Information For Matn2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O08746

Gene ID

17181

Gene Name

Matn2

Full Name

Matrilin-2

Weight

106.748kDa

Alternative Names

MATN2; matrilin 2; Matrilin2; Matrilin-2 Matn2|Crtm, Crtm2, matrilin-2|matrilin 2|matrilin-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Matn2, check out the Matn2 Infographic

Matn2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Matn2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse Matrilin-2 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-05-20

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-05-20

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Matrilin-2 level in serum.

W. Rodriguez

Verified customer

Asked: 2019-11-13

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-13

Question

Q: is it okay to use citrate plasma as samples in Mouse Matrilin-2 Picokine® ELISA Kit (Catalog # EK2021)?

C. Parker

Verified customer

Asked: 2019-10-05

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Matrilin-2 causing degradation of its protein structure. Matrilin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Matrilin-2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-10-05

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

T. Maharaj

Verified customer

Asked: 2019-05-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-05-10

Question

Q: What is the optimal O.D. value for Matrilin-2 ELISA kit? I used your Matrilin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Matrilin-2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Matrilin-2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Matrilin-2 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-12-05

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-03-23

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-03-23

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

D. Walker

Verified customer

Asked: 2018-03-18

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-03-18

Question

Q: which procedure should I follow in order to thaw whole blood sample for Matrilin-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-03-08

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Matrilin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-03-08

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