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Product Info Summary
| SKU: | EK2021 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Matrilin-2 ELISA Kit PicoKine®
View all Matrilin-2 ELISA kits
SKU/Catalog Number
EK2021
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Matrilin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Matn2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Matrilin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2021)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: R24-R956
Sensitivity
<50 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2021 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2021 is reactive to MATN2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2021 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Matrilin-2
Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 15; 15 B3.1. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2021-CAP | Anti-Mouse MATN2 Pre-coated 96-well strip microplate | 1 |
| EK2021-ST | Mouse MATN2 Standard | 2 vials, 10 ng/tube |
| EK2021-DA | Mouse MATN2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.044 | 0.132 | 0.212 | 0.346 | 0.591 | 1.063 | 1.659 | 2.189 |
Data Example Images
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Mouse Matrilin-2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Matrilin-2, Dilution ratio of 1:5, concentration in serum and plasma is 300-3200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Matrilin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 178 | 657 | 2396 | 181 | 685 | 2417 |
| Standard deviation | 8.01 | 37.45 | 110.22 | 9.23 | 40.42 | 178.86 |
| CV (%) | 4.5% | 5.7% | 4.6% | 5.1% | 5.9% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Matrilin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 178 | 194 | 184 | 185 | 185 | 5.71 | 3.0% |
| Sample 2 | 657 | 670 | 600 | 591 | 629 | 34.45 | 5.4% |
| Sample 3 | 2396 | 2289 | 2414 | 2332 | 2357 | 50.04 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For MATN2 (Source: Uniprot.Org, NCBI)
Gene Name
MATN2
Full Name
Matrilin-2
Weight
106.837kDa
Alternative Names
MATN2; matrilin 2; Matrilin2; Matrilin-2 MATN2 matrilin 2 matrilin-2|testis tissue sperm-binding protein Li 94mP
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MATN2, check out the MATN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MATN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Matrilin-2 ELISA Kit PicoKine® (EK2021)
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No publications found for EK2021
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Mouse Matrilin-2 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-05-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-20
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Matrilin-2 level in serum.
W. Rodriguez
Verified customer
Asked: 2019-11-13
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-13
Question
Q: is it okay to use citrate plasma as samples in Mouse Matrilin-2 Picokine® ELISA Kit (Catalog # EK2021)?
C. Parker
Verified customer
Asked: 2019-10-05
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Matrilin-2 causing degradation of its protein structure. Matrilin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Matrilin-2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
T. Maharaj
Verified customer
Asked: 2019-05-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-05-10
Question
Q: What is the optimal O.D. value for Matrilin-2 ELISA kit? I used your Matrilin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Matrilin-2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Matrilin-2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Matrilin-2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-12-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Walker
Verified customer
Asked: 2018-03-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for Matrilin-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-03-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Matrilin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-03-08
