Overview
| Product Name |
Mouse MMP-12 ELISA Kit PicoKine™
See all MMP-12 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0996 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Mouse MMP12 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP12 tissue specificity: |
| Cite This Product |
Mouse MMP-12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0996)
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum and plasma(heparin). |
| Sensitivity |
<10pg/ml |
| Assay Range |
62.5pg/ml-4000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: A18-C462 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Mmp12 (Source: Uniprot.org, NCBI)
| Uniprot Id | P34960 |
|---|
| NCBI Gene Id | 17381 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Mmp12 |
|---|
| Protein Name | Macrophage metalloelastase |
|---|
| Superfamily | peptidase M10A family |
|---|
| Alternative Names | MMP-12|EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 |
|---|
| Molecular Weight | 54971 |
|---|
| Post Tranlational Modifications | Cleavage; Phosphorylation; Oxidation; Acetylation; Dephosphorylation |
|---|
| Gene Location | 9 A1|9 2.46 cM, on Chromasome 9, gene sequence: |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Mmp12 (Source: Uniprot.org, NCBI)
| Protein Function | May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity). . |
|---|
| Related Diseases | Inflammation; Lung Diseases; Malignant Neoplasms; Neoplasms; Pulmonary Emphysema; Chronic Obstructive Airway Disease; Lung Diseases, Obstructive; Carcinoma; Cell Invasion; Neoplasm Metastasis; Fibrosis; Tumor Angiogenesis; Atherosclerosis; Neoplasm Invasiveness; Inflammatory Response; Malignant Squamous Cell Neoplasm; Pathologic Neovascularization; Aneurysm; Asthma; Lung Neoplasms |
|---|
| Related Pathways | Pathogenesis; Angiogenesis; Inflammatory Response; Tissue Remodeling; Proteolysis; Localization; Secretion; Cell Migration; Cell Proliferation; Macrophage Activation; Immune Response; Wound Healing; Cell Adhesion; Cell Differentiation; Cell Cycle; Cell Growth; Dna Replication; Dedifferentiation; Aging; Tube Formation |
|---|
| Background | Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling. |
|---|
| Scientific References | PMID: 1537850 by Shapiro S.D., et al. Molecular cloning, chromosomal localization, and bacterial expression of a murine macrophage metalloelastase. |
|---|
Kit Components And Assay QC Data Details Of Mouse MMP-12 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0996-CAP | 96-well plate precoated with anti-Mouse MMP-12 antibody | 1 |
| EK0996-ST | lyophilized recombinant Mouse MMP-12 standard | 10ng/tube |
| EK0996-DA | biotinylated anti-Mouse MMP-12 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0996 Mouse MMP-12 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.087 | 0.148 | 0.203 | 0.281 | 0.516 | 0.820 | 1.537 | 2.195 |
We measured random samples of Mouse MMP-12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 127 | 628 | 1430 | 134 | 684 | 1370 |
| Standard deviation | 9.27 | 46.47 | 100.1 | 11.12 | 57.45 | 97.27 |
| CV(%) | 7.3% | 7.4% | 7% | 8.3% | 8.4% | 7.1% |
We measured the performance consistency of Mouse MMP-12 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 127 | 129 | 117 | 123 | 124 | 4.58 | 3.6% |
| Sample 2 | 628 | 722 | 630 | 605 | 646 | 44.82 | 6.9% |
| Sample 3 | 1430 | 1243 | 1362 | 1284 | 1329 | 71.95 | 5.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all MMP-12 primary antibodies,
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
| Vaz M, Rajasekaran S, Potteti Hr, Reddy Sp. Am J Respir Cell Mol Biol. 2015 Jul;53(1):125-34. Doi: 10.1165/Rcmb.2014-0118Oc. Myeloid-Specific Fos-Related Antigen-1 Regulates Cigarette Smoke-Induced Lung Inflammation, Not Emphysema, In Mice. |
PubMed ID 25489966 |
| Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection |
PubMed ID 26126526 |
| Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection |
PubMed ID 26676790 |
