Mouse MMP-12 ELISA Kit PicoKine™

Mouse MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0996
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse MMP-12 ELISA Kit PicoKine™

See all MMP-12 products

SKU/Catalog Number

EK0996

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MMP-12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0996)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-C462

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0996 is reactive to Mmp12 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0996 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0996-CAP Anti-Mouse Mmp12 Pre-coated 96-well strip microplate 1
EK0996-ST Mouse Mmp12 Standard 2 vials, 10 ng/tube
EK0996-DA Mouse Mmp12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0490.1320.2110.3670.7001.0251.4341.891

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-12, Dilution ratio of 1:1, concentration in serum is around 100pg/ml.

Some articles we found to cite concentrations of MMP-12 in samples: 25652021 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MMP-12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12762814301346841370
Standard deviation9.2746.47100.111.1257.4597.27
CV (%)7.3%7.4%7%8.3%8.4%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11271291171231244.583.6%
Sample 262872263060564644.826.9%
Sample 31430124313621284132971.955.4%
*number of samples for each test n=16.

Gene/Protein Information For Mmp12 (Source: Uniprot.Org, NCBI)

Uniprot ID

P34960

Gene ID

17381

Gene Name

Mmp12

Full Name

Macrophage metalloelastase

Weight

54.971kDa

Superfamily

peptidase M10A family

Alternative Names

EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 Mmp12|AV378681, MME, MMP1, Mmel|matrix metallopeptidase 12|macrophage metalloelastase|macrophage elastase|matrix metalloproteinase 12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp12, check out the Mmp12 Infographic

Mmp12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0996 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection

Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection

Vaz M, Rajasekaran S, Potteti Hr, Reddy Sp. Am J Respir Cell Mol Biol. 2015 Jul;53(1):125-34. Doi: 10.1165/Rcmb.2014-0118Oc. Myeloid-Specific Fos-Related Antigen-1 Regulates Cigarette Smoke-Induced Lung Inflammation, Not Emphysema, In Mice.

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9 Customer Q&As for Mouse MMP-12 ELISA Kit PicoKine™

Question

Can EK0996 cross react with rat? If you have tested this, what samples types for tested?

Verified customer

Asked: 2020-11-09

Answer

Our lab hasn't validated cross reactivity with rats. The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.

Boster Scientific Support

Answered: 2020-11-10

Question

Can EK0996 detect total MMP-12, just active MMP-12 or just Pro-MMP-12?

Verified customer

Asked: 2020-10-15

Answer

The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.

Boster Scientific Support

Answered: 2020-10-16

Question

Q: how to analyze ELISA data? I have performed MMP-12 level in serum.

Verified Customer

Verified customer

Asked: 2020-09-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

W. Wang

Verified customer

Asked: 2020-06-12

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Mehta

Verified customer

Asked: 2019-06-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-06-27

Question

Q: Can MMP-12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-04-20

Question

Would this mouse ELISA work for human MMP-12? Do you sell the MMP-12 detection antibody that is specific for the human form only?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

No, the mouse kit would not work for human MMP-12 because there is no cross reactivity between the two species. Yes, although not in our product catalog at this time, you can buy the MMP-12 detection antibody alone from the kit.

Boster Scientific Support

Answered: 2018-02-05

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-10-05

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-10-05

Question

Q: is it okay to use citrate plasma as samples in Mouse MMP-12 Picokine® ELISA Kit (Catalog # EK0996)?

V. Varma

Verified customer

Asked: 2016-03-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MMP-12 causing degradation of its protein structure. MMP-12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-12 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-03-09

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