Overview
| Product Name |
Mouse MMP-12 ELISA Kit PicoKine™
See more MMP-12 products |
| Catalog# |
EK0996 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp12 in cell culture supernatants, serum and plasma (heparin).. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse MMP-12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0996)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma (heparin). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
62.5 pg/ml - 4,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: A18-C462 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Mmp12 (Source: Uniprot.org, NCBI)
| Uniprot Id | P34960 |
|---|
| NCBI Gene Id | 17381 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Mmp12 |
|---|
| Protein Name | Macrophage metalloelastase |
|---|
| Superfamily | peptidase M10A family |
|---|
| Alternative Names | MMP-12|EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 |
|---|
| Molecular Weight | 54971 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Mmp12, check out the Mmp12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Mmp12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Mmp12 infographic
Kit Components And Assay QC Data Details Of Mouse MMP-12 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0996-CAP |
Anti-Mouse Mmp12 Pre-coated 96-well strip microplate | 1 |
| EK0996-ST | Mouse Mmp12 Standard | 2 vials, 10 ng/tube |
| EK0996-DA | Mouse Mmp12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0996 Mouse MMP-12 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.049 | 0.132 | 0.211 | 0.367 | 0.700 | 1.025 | 1.434 | 1.891 |
We measured random samples of Mouse MMP-12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 127 | 628 | 1430 | 134 | 684 | 1370 |
| Standard deviation | 9.27 | 46.47 | 100.1 | 11.12 | 57.45 | 97.27 |
| CV (%) | 7.3% | 7.4% | 7% | 8.3% | 8.4% | 7.1% |
We measured the performance consistency of Mouse MMP-12 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 127 | 129 | 117 | 123 | 124 | 4.58 | 3.6% |
| Sample 2 | 628 | 722 | 630 | 605 | 646 | 44.82 | 6.9% |
| Sample 3 | 1430 | 1243 | 1362 | 1284 | 1329 | 71.95 | 5.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
| Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection |
PubMed ID 26676790 |
| Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection |
PubMed ID 26126526 |
| Vaz M, Rajasekaran S, Potteti Hr, Reddy Sp. Am J Respir Cell Mol Biol. 2015 Jul;53(1):125-34. Doi: 10.1165/Rcmb.2014-0118Oc. Myeloid-Specific Fos-Related Antigen-1 Regulates Cigarette Smoke-Induced Lung Inflammation, Not Emphysema, In Mice. |
PubMed ID 25489966 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0996 Mouse MMP-12 ELISA Kit PicoKine™
9 Related Questions
Question
Would this mouse ELISA work for human MMP-12? Do you sell the MMP-12 detection antibody that is specific for the human form only?
Verified Customer
Asked: 2018-02-05
Answer
No, the mouse kit would not work for human MMP-12 because there is no cross reactivity between the two species. Yes, although not in our product catalog at this time, you can buy the MMP-12 detection antibody alone from the kit.
Boster Scientific Support
Answered: 2018-02-05
Question
Q: how to analyze ELISA data? I have performed MMP-12 level in serum.
Verified Customer
Asked: 2020-09-09
Boster Scientific Support
Answered: 2020-09-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
W. Wang
Asked: 2020-06-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Mehta
Asked: 2019-06-27
Boster Scientific Support
Answered: 2019-06-27
Question
Q: Can MMP-12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2018-04-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-04-20
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2016-10-05
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-10-05
Question
Q: is it okay to use citrate plasma as samples in Mouse MMP-12 Picokine® ELISA Kit (Catalog # EK0996)?
V. Varma
Asked: 2016-03-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MMP-12 causing degradation of its protein structure. MMP-12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-12 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-03-09
Question
Can EK0996 cross react with rat? If you have tested this, what samples types for tested?
Answer
Our lab hasn't validated cross reactivity with rats. The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.
Boster Scientific Support
Answered: 2020-11-10
Question
Can EK0996 detect total MMP-12, just active MMP-12 or just Pro-MMP-12?
Answer
The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.
Boster Scientific Support
Answered: 2020-10-16
