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Product Info Summary
| SKU: | EK1266 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, tissue, serum and plasma (heparin or EDTA) |
Product info
Product Name
Mouse Myostatin/GDF8 ELISA Kit PicoKine®
View all GDF-8/Myostatin ELISA kits
SKU/Catalog Number
EK1266
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Myostatin/GDF8 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Myostatin/GDF8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1266)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D268-S376
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1266 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1266 is reactive to MSTN in Mouse samples
Validated Sample Types
cell culture supernatants, tissue, serum and plasma (heparin or EDTA)
Application Guarantee
EK1266 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDF-8/Myostatin
Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1266-CAP | Anti-Mouse MSTN Pre-coated 96-well strip microplate | 1 |
| EK1266-ST | Mouse MSTN Standard | 2 vials, 10 ng/tube |
| EK1266-DA | Mouse MSTN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.076 | 0.150 | 0.185 | 0.292 | 0.520 | 0.839 | 1.496 | 2.092 |
Data Example Images
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Mouse Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GDF-8/Myostatin, Dilution ratio of 1:50, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Myostatin/GDF8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 51 | 227 | 971 | 59 | 238 | 984 |
| Standard deviation | 2.04 | 11.12 | 54.38 | 4.01 | 12.38 | 58.06 |
| CV (%) | 4.0% | 4.9% | 5.6% | 6.8% | 5.2% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDF-8/Myostatin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 55 | 46 | 51 | 50 | 3.19 | 6.3% |
| Sample 2 | 227 | 216 | 255 | 226 | 231 | 14.50 | 6.2% |
| Sample 3 | 971 | 973 | 959 | 989 | 973 | 10.67 | 1.0% |
Protein Target Info & Infographic
Gene/Protein Information For MSTN (Source: Uniprot.Org, NCBI)
Gene Name
MSTN
Full Name
Growth/differentiation factor 8
Weight
42.75kDa
Superfamily
TGF-beta family
Alternative Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin MSTN GDF8, MSLHP myostatin growth/differentiation factor 8
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MSTN, check out the MSTN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Myostatin/GDF8 ELISA Kit PicoKine® (EK1266)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse Myostatin/GDF8 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-11-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-11-30
Question
Q: can you recommend the dilution ratio of serum samples for detection of GDF-8 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GDF-8 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-04-17
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GDF-8 using the EK1266 Mouse GDF-8 Picokine® ELISA Kit?
A. Sunder
Verified customer
Asked: 2019-07-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-07-27
Question
Q: how can I thaw whole blood sample for GDF-8 ELISA after freezing?
D. Anderson
Verified customer
Asked: 2018-10-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GDF-8 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-21
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-09-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-09-10
Question
Q: if the enzyme conjugated GDF-8 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GDF-8 antigen?
Verified Customer
Verified customer
Asked: 2016-08-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-08-06
