Rat Myostatin/GDF8 ELISA Kit PicoKine™

Rat Myostatin/GDF8 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1267
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, tissue, serum and plasma (heparin or EDTA)

Product Name

Rat Myostatin/GDF8 ELISA Kit PicoKine™

See all GDF-8/Myostatin products

SKU/Catalog Number

EK1267

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Myostatin/GDF8 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Myostatin/GDF8 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1267)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D268-S376

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1267 is reactive to MSTN in Rat samples

Validated Sample Types

cell culture supernatants, tissue, serum and plasma (heparin or EDTA)

Application Guarantee

EK1267 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1267-CAP Anti-Rat MSTN Pre-coated 96-well strip microplate 1
EK1267-ST Rat MSTN Standard 2 vials, 10 ng/tube
EK1267-DA Rat MSTN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0760.1500.1850.2920.5200.8391.4962.292

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect GDF-8/Myostatin, Dilution ratio of 1:10, concentration in serum is around 5ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Myostatin/GDF8 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5522884560239884
Standard deviation2.3114.3638.033.1815.3063.65
CV (%)4.2%6.3%4.5%5.3%6.4%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GDF-8/Myostatin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155535052521.803.4%
Sample 222826122826424517.287.0%
Sample 384578883884582923.842.8%
*number of samples for each test n=16.

Gene/Protein Information For MSTN (Source: Uniprot.Org, NCBI)

Uniprot ID

O35312

Gene ID

29152

Gene Name

MSTN

Full Name

Growth/differentiation factor 8

Weight

42.829kDa

Superfamily

TGF-beta family

Alternative Names

GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin Mstn|Gdf8|myostatin|growth/differentiation factor 8|GDF-8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MSTN, check out the MSTN Infographic

MSTN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Rat Myostatin/GDF8 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for GDF-8 ELISA kit? I performed your GDF-8 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain GDF-8 even though the O.D. values are not very high?

J. Collins

Verified customer

Asked: 2020-02-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GDF-8 ELISA kit to have sensitivity of <.10pg/ml, that means the minimum amount of GDF-8 that can be declared/interpreted as positive by the above standard is <.10pg/ml.

Boster Scientific Support

Answered: 2020-02-04

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GDF-8 using the EK1267 Rat GDF-8 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-06-07

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-03-09

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-03-09

Question

Q: is it okay to use citrate plasma as samples in Rat GDF-8 Picokine® ELISA Kit (Catalog # EK1267)?

L. Banerjee

Verified customer

Asked: 2018-12-31

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of GDF-8 causing degradation of its protein structure. GDF-8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GDF-8 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-31

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. King

Verified customer

Asked: 2018-12-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-08

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