Mouse Neurotrophin-3 ELISA Kit PicoKine™

Mouse Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ntf3 in cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0473
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse Neurotrophin-3 ELISA Kit PicoKine™

See all NT-3 products

SKU/Catalog Number

EK0473

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ntf3 in cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Neurotrophin-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0473)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y139-T257

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.

Reactive Species

EK0473 is reactive to Ntf3 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0473 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0473-CAP Anti-Mouse Ntf3 Pre-coated 96-well strip microplate 1
EK0473-ST Mouse Ntf3 Standard 2 vials, 10 ng/tube
EK0473-DA Mouse Ntf3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0030.0560.0970.2030.3880.6911.2861.906

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect NT-3, Dilution ratio of 1:1, concentration in serum is around 2pg/ml.

Some articles we found to cite concentrations of NT-3 in samples: 22037732 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Neurotrophin-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3113153231208563
Standard deviation2.292.8529.792.859.3633.78
CV (%)7.4%4%5.6%9.2%4.5%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of NT-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131313329311.414.5%
Sample 21951892011951954.242.1%
Sample 353246645847848328.895.9%
*number of samples for each test n=16.

Gene/Protein Information For Ntf3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20181

Gene ID

18205

Gene Name

Ntf3

Full Name

Neurotrophin-3

Weight

29.588kDa

Superfamily

NGF-beta family

Alternative Names

HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3 Ntf3|AI316846, AI835689, HDNF, NGF-2, NT, NT-, Nt3, Ntf-, Ntf-3|neurotrophin 3|neurotrophin-3|nerve growth factor 2|neurotrophic factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ntf3, check out the Ntf3 Infographic

Ntf3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0473 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhao Z, Wang Y, Peng J, Ren Z, Zhang L, Guo Q, Xu W, Lu S. Cell Transplant. 2014 Jan;23(1):97-110. Doi: 10.3727/096368912X658845. Epub 2012 Nov 1. Improvement In Nerve Regeneration Through A Decellularized Nerve Graft By Supplementation With Bone ...

Wang Y, Li J, Kong P, Zhao S, Yang H, Chen C, Yan J. Spine (Phila Pa 1976). 2015 Jan 15;40(2):95-101. Doi: 10.1097/Brs.0000000000000694. Enhanced Expression Of Neurotrophic Factors In The Injured Spinal Cord Through Vaccination With Myelin Basic P...

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6 Customer Q&As for Mouse Neurotrophin-3 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-11-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-11-12

Question

Q: What is the optimal O.D. value for NT-3 ELISA kit? I used your NT-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain NT-3 even though the O.D. values are not very high?

P. Phillips

Verified customer

Asked: 2020-03-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this NT-3 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of NT-3 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: can you recommend the dilution ratio of serum samples for detection of NT-3 in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

P. Phillips

Verified customer

Asked: 2018-10-31

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the NT-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-10-31

Question

Q: how do I analyze ELISA data? I have obtained NT-3 level in serum.

Verified Customer

Verified customer

Asked: 2018-08-04

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-04

Question

Q: if the enzyme conjugated NT-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NT-3 antigen?

T. Mangal

Verified customer

Asked: 2017-10-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-10-10

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-03-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-03-29

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