Mouse NGF/NGF Beta ELISA Kit PicoKine™

Mouse NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ngf in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 7 publication(s).

Product Info Summary

SKU: EK0470
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse NGF/NGF Beta ELISA Kit PicoKine™

See all beta-NGF products

SKU/Catalog Number

EK0470

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ngf in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse NGF/NGF Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0470)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S122-G241

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.

Reactive Species

EK0470 is reactive to Ngf in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0470 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0470-CAP Anti-Mouse Ngf Pre-coated 96-well strip microplate 1
EK0470-ST Mouse Ngf Standard 2 vials, 10 ng/tube
EK0470-DA Mouse Ngf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0330.0960.1380.2380.4430.7401.2391.852

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse NGF/NGF Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9923591099244967
Standard deviation6.4317.3970.076.4318.0580.26
CV (%)6.5%7.4%7.7%6.5%7.4%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 199878382876.757.7%
Sample 223523123020322412.695.6%
Sample 391088789893690718.222%
*number of samples for each test n=16.

Gene/Protein Information For Ngf (Source: Uniprot.Org, NCBI)

Uniprot ID

P01139

Gene ID

18049

Gene Name

Ngf

Full Name

Beta-nerve growth factor

Weight

27.077kDa

Superfamily

NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 Ngf|Ngfb, beta-NGF|nerve growth factor|beta-nerve growth factor|nerve growth factor, beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ngf, check out the Ngf Infographic

Ngf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ngf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0470 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Magnetic NGF-releasing PLLA/iron oxide nanoparticles direct extending neurites and preferentially guide neurites along aligned electrospun microfibers.

The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury

Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury

Wang L, Zhao H, Fan Yp, Gong Hy, Li M, Qi F, Liu Y. Chin J Integr Med. 2010 Apr;16(2):167-72. Doi: 10.1007/S11655-010-0167-Z. Epub 2010 May 16. Research On The Mechanism Of Zuogui Pill And Yougui Pill In Promoting Axonal Regeneration In Model Rats...

Fink Dm, Connor Al, Kelley Pm, Steele Mm, Hollingsworth Ma, Tempero Rm. Plos One. 2014 Nov 10;9(11):E112737. Doi: 10.1371/Journal.Pone.0112737. Ecollection 2014. Nerve Growth Factor Regulates Neurolymphatic Remodeling During Corneal Inflammation A...

Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.

Tan T, Xie J, Liu T, Chen X, Zheng X, Tong Z, Tian X. Exp Gerontol. 2013 Aug;48(8):786-94. Doi: 10.1016/J.Exger.2013.05.001. Epub 2013 May 9. Low-Frequency (1 Hz) Repetitive Transcranial Magnetic Stimulation (Rtms) Reverses A??(1-42)-Mediated Memo...

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12 Customer Q&As for Mouse NGF/NGF Beta ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for beta-NGF ELISA after freezing?

M. Singh

Verified customer

Asked: 2020-12-09

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test beta-NGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-12-09

Question

Q: if the enzyme conjugated beta-NGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the beta-NGF antigen?

Verified Customer

Verified customer

Asked: 2020-07-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-07-27

Question

Q: What is the optimal O.D. value for beta-NGF ELISA kit? I used your beta-NGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain beta-NGF even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-06-23

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this beta-NGF ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of beta-NGF that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2020-06-23

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Taylor

Verified customer

Asked: 2020-05-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis beta-NGF using the EK0470 Mouse beta-NGF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2020-04-17

Question

Q: Can beta-NGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-16

Question

Are you selling the coated microplates of EK0470 kit separately if damage is sustained during shipment?

Verified customer

Asked: 2019-08-08

Answer

Yes, we are able to sell the coated microplates of the Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 separately. You may purchase using the sku EK0470-CAP and also include the lot number on the Purchase Order.

Boster Scientific Support

Answered: 2019-08-09

Question

Was thimerosal used in any of the reagents for the EK0470 kit?

Verified customer

Asked: 2019-07-30

Answer

The Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 does not contain thimerosal in any of its reagents.

Boster Scientific Support

Answered: 2019-07-30

Question

Was thimerosal used in any of the reagents for the EK0470 kit?

Verified customer

Asked: 2019-07-30

Answer

The Mouse NGF/NGF Beta ELISA Kit PicoKine EK0470 does not contain thimerosal in any of its reagents.

Boster Scientific Support

Answered: 2019-07-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Baker

Verified customer

Asked: 2018-06-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-16

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-02-17

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

L. Martin

Verified customer

Asked: 2017-12-01

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-12-01

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