Mouse NGF/NGF Beta ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse NGF/NGF beta. 96wells/kit, with removable strips. Cited in 6 publication(s).
Mouse NGF/NGF Beta ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Product Name||Mouse NGF/NGF Beta ELISA Kit PicoKine™
See all Ngf primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Beta Ngf/NGF with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Beta Ngf/NGF tissue specificity:|
|Cite This Product||Mouse NGF/NGF Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0470)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Immunogen||Expression system for standard: NSO; Immunogen sequence: S122-G241|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Beta-nerve growth factor|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Mouse NGF/NGF Beta ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0470-CAP||96-well plate precoated with anti-Mouse Ngf antibody||1|
|EK0470-ST||lyophilized recombinant Mouse Ngf standard||10ng/tube|
|EK0470-DA||biotinylated anti-Mouse Ngf antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0470 Mouse NGF/NGF Beta ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse NGF/NGF Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse NGF/NGF Beta ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 6
|Tan T, Xie J, Liu T, Chen X, Zheng X, Tong Z, Tian X. Exp Gerontol. 2013 Aug;48(8):786-94. Doi: 10.1016/J.Exger.2013.05.001. Epub 2013 May 9. Low-Frequency (1 Hz) Repetitive Transcranial Magnetic Stimulation (Rtms) Reverses A??(1-42)-Mediated Memo...||PubMed ID 23665072|
|Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.||PubMed ID 26170829|
|Fink Dm, Connor Al, Kelley Pm, Steele Mm, Hollingsworth Ma, Tempero Rm. Plos One. 2014 Nov 10;9(11):E112737. Doi: 10.1371/Journal.Pone.0112737. Ecollection 2014. Nerve Growth Factor Regulates Neurolymphatic Remodeling During Corneal Inflammation A...||PubMed ID 25383879|
|Wang L, Zhao H, Fan Yp, Gong Hy, Li M, Qi F, Liu Y. Chin J Integr Med. 2010 Apr;16(2):167-72. Doi: 10.1007/S11655-010-0167-Z. Epub 2010 May 16. Research On The Mechanism Of Zuogui Pill And Yougui Pill In Promoting Axonal Regeneration In Model Rats...||PubMed ID 20473744|
|Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury||PubMed ID 27778376|
|Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury||PubMed ID 29274773|
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Note: sample size takes longer to process, average 5-7 days.
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