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Mouse NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ngf in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 1pg/ml. Cited in 7 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Assay Range:||31.2 pg/ml - 2,000 pg/ml|
|Product Name||Mouse NGF/NGF Beta ELISA Kit PicoKine™
See more beta-NGF products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Mouse NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ngf in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 1pg/ml.|
|Cite This Product||Mouse NGF/NGF Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0470)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Assay Range||31.2 pg/ml - 2,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: S122-G241|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||18049|
|Species Of This Entry||Mouse|
|Protein Name||Beta-nerve growth factor|
|Alternative Names||beta-NGF|Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ngf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0470-CAP||Anti-Mouse Ngf Pre-coated 96-well strip microplate||1|
|EK0470-ST||Mouse Ngf Standard||2 vials, 10 ng/tube|
|EK0470-DA||Mouse Ngf Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0470 Mouse NGF/NGF Beta ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse NGF/NGF Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Magnetic NGF-releasing PLLA/iron oxide nanoparticles direct extending neurites and preferentially guide neurites along aligned electrospun microfibers.||PubMed ID 26322376|
|The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury||PubMed ID 29516546|
|Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury||PubMed ID 27778376|
|Wang L, Zhao H, Fan Yp, Gong Hy, Li M, Qi F, Liu Y. Chin J Integr Med. 2010 Apr;16(2):167-72. Doi: 10.1007/S11655-010-0167-Z. Epub 2010 May 16. Research On The Mechanism Of Zuogui Pill And Yougui Pill In Promoting Axonal Regeneration In Model Rats...||PubMed ID 20473744|
|Fink Dm, Connor Al, Kelley Pm, Steele Mm, Hollingsworth Ma, Tempero Rm. Plos One. 2014 Nov 10;9(11):E112737. Doi: 10.1371/Journal.Pone.0112737. Ecollection 2014. Nerve Growth Factor Regulates Neurolymphatic Remodeling During Corneal Inflammation A...||PubMed ID 25383879|
|Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.||PubMed ID 26170829|
|Tan T, Xie J, Liu T, Chen X, Zheng X, Tong Z, Tian X. Exp Gerontol. 2013 Aug;48(8):786-94. Doi: 10.1016/J.Exger.2013.05.001. Epub 2013 May 9. Low-Frequency (1 Hz) Repetitive Transcranial Magnetic Stimulation (Rtms) Reverses A??(1-42)-Mediated Memo...||PubMed ID 23665072|
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Comprehensive info on Ngf, beautifully presented.Open Infographic
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