Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine™

Mouse Osteoadherin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Omd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2018
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine™

See all Osteoadherin/OSAD/OMD products

SKU/Catalog Number

EK2018

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Osteoadherin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Omd in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2018)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I423

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2018 is reactive to Omd in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2018 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2018-CAP Anti-Mouse Omd Pre-coated 96-well strip microplate 1
EK2018-ST Mouse Omd Standard 2 vials, 10 ng/tube
EK2018-DA Mouse Omd Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.1040.1470.1810.2690.4240.7121.2862.230

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Osteoadherin/OSAD/OMD, Dilution ratio of 1:1, concentration in serum is 582-3124pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2791140446030811904553
Standard deviation13.3949.02182.8620.3369.02346.03
CV (%)4.8%4.3%4.1%6.6%5.8%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Osteoadherin/OSAD/OMD in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12792802862932845.591.9%
Sample 211409831062973103967.496.4%
Sample 344604445395144754332220.655.0%
*number of samples for each test n=16.

Gene/Protein Information For Omd (Source: Uniprot.Org, NCBI)

Uniprot ID

O35103

Gene ID

27047

Gene Name

Omd

Full Name

Osteomodulin

Weight

49.745kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; OMD; OSAD; osteoadherin proteoglycan; Osteoadherin; osteomodulin; SLRR2C; SLRR2Costeoadherin Omd|O, OSAD, SL, SLRR2C|osteomodulin|osteomodulin|KSPG osteomodulin|keratan sulfate proteoglycan osteomodulin|osteoadherin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Omd, check out the Omd Infographic

Omd infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Omd: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine™

Question

Q: Can Osteoadherin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. Brown

Verified customer

Asked: 2020-08-22

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-08-22

Question

Q: What is the optimal O.D. value for Osteoadherin ELISA kit? I performed your Osteoadherin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Osteoadherin even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-07-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Osteoadherin ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Osteoadherin that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2020-07-07

Question

Q: if the enzyme conjugated Osteoadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Osteoadherin antigen?

Verified Customer

Verified customer

Asked: 2020-06-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-06-12

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Osteoadherin using the EK2018 Mouse Osteoadherin Picokine® ELISA Kit?

J. Carter

Verified customer

Asked: 2019-06-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-06-24

Question

Q: can I use citrate plasma as samples in Mouse Osteoadherin Picokine® ELISA Kit (Catalog # EK2018)?

Verified Customer

Verified customer

Asked: 2018-07-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Osteoadherin causing degradation of its protein structure. Osteoadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Osteoadherin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-07-10

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Osteoadherin in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

C. King

Verified customer

Asked: 2018-04-03

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Osteoadherin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-03

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