Click image to see more details
Product Info Summary
| SKU: | EK1945 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse PLA2G2A ELISA Kit PicoKine®
SKU/Catalog Number
EK1945
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse PLA2G2A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pla2g2a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse PLA2G2A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1945)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: N22-C146
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1945 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1945 is reactive to PLA2G2A in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1945 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PLA2G2A
Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1945-CAP | Anti-Mouse PLA2G2A Pre-coated 96-well strip microplate | 1 |
| EK1945-ST | Mouse PLA2G2A Standard | 2 vials, 10 ng/tube |
| EK1945-DA | Mouse PLA2G2A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.051 | 0.185 | 0.312 | 0.515 | 0.824 | 1.142 | 1.448 | 1.722 |
Data Example Images
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Mouse PLA2G2A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PLA2G2A, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PLA2G2A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 56 | 234 | 938 | 58 | 242 | 951 |
| Standard deviation | 2.69 | 13.34 | 43.15 | 2.96 | 14.28 | 60.86 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PLA2G2A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 59 | 55 | 62 | 58 | 2.73 | 4.7% |
| Sample 2 | 234 | 232 | 234 | 234 | 233 | 0.86 | 0.3% |
| Sample 3 | 938 | 983 | 1055 | 1031 | 1001 | 45.01 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For PLA2G2A (Source: Uniprot.Org, NCBI)
Gene Name
PLA2G2A
Full Name
Phospholipase A2, membrane associated
Weight
16.083kDa
Superfamily
phospholipase A2 family
Alternative Names
EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; Mom1; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLAS1; RASF-A; sPLA2 PLA2G2A MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2 phospholipase A2 group IIA phospholipase A2, membrane associated|GIIC sPLA2|NPS-PLA2|group IIA phospholipase A2|non-pancreatic secretory phospholipase A2|phosphatidylcholine 2-acylhydrolase 2A|phospholipase A2, group IIA (platelets, synovial fluid)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLA2G2A, check out the PLA2G2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLA2G2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse PLA2G2A ELISA Kit PicoKine® (EK1945)
Hello CJ!
EK1945 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Moslea Herba flavonoids alleviated influenza A virus-induced pulmonary endothelial barrier disruption via suppressing NOX4/NF-κB/MLCK pathway
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Mouse PLA2G2A ELISA Kit PicoKine®
Question
We intend to use mouse serum of C57BL/6 as a negative control. Do you think EK1945 cannot recognize mouse strains C57BL/6?
Verified customer
Asked: 2021-01-06
Answer
The immunogen sequence of both the capture antibody and detection antibody is N22-C146. Our lab has never tested mouse serum of C57BL/6 using the Mouse PLA2G2A ELISA Kit PicoKine™ (EK1945) and we are uncertain if the sample could be used as a negative control. We suggest to run a pilot test to see if PLA2G2A could be tested in mouse serum of C57BL/6.
Boster Scientific Support
Answered: 2021-01-06
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PLA2G2A using the EK1945 Mouse PLA2G2A Picokine® ELISA Kit?
K. Turner
Verified customer
Asked: 2020-05-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-05-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-03
Question
Q: Can PLA2G2A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Hall
Verified customer
Asked: 2019-10-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-10-22
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-07-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-07-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Mitchell
Verified customer
Asked: 2018-06-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-24
Question
Q: how to analyze ELISA data? I measured PLA2G2A level in serum.
G. Singh
Verified customer
Asked: 2018-05-26
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-26
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
L. Bhasin
Verified customer
Asked: 2017-04-10
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-04-10
