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- Table of Contents
LGR5 antibody (M00239) performs well in Flow Cytometry, IP, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Western blot analysis of GPR49 expression in Human fetal skeletal muscle lysate.
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Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
LGR5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, WB |
Tested Applications
LGR5 antibody was positively detected by WB in:
Human fetal skeletal muscle
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IP
FC
Human
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
View all LGR5 Antibodies
View all LGR5 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CHF-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LGR5/GPR49
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
100.0 kDa
Cite This Product
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00239)
Protocols
Western Blot protocol for LGR5 antibody
Publications
Application:
Species:
Wumei Wan ameliorates ulcerative colitis in rats by modulating the inflammation-pyroptosis-intestinal stem cell axis
Published: 2025-09-24
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 5.40
Species: Rat
PMID: 41005471
Integration of Multi-Omics and Network Pharmacology Analysis Reveals the Mechanism of Qingchang Huashi Jianpi Bushen Formula in Repairing the Epithelial Barrier of Ulcerative Colitis
Published: 2025-05-13
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 40386180
Metal-organic-framework-based sitagliptin-release platform for multieffective radiation-induced intestinal injury targeting therapy and intestinal flora protective capabilities
Published: 2024-10-16
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: IHC
Impact Factor: 10.60
Species: Mouse
PMID: 39415273
Sitagliptin Alleviates Radiation-Induced Intestinal Injury by Activating NRF2-Antioxidant Axis, Mitigating NLRP3 Inf--lammasome Activation, and Reversing Gut Microbiota Disorder
Published: 2022-05-17
Journal: Oxidative Medicine and Cellular Longevity
Application: IHC
Impact Factor: 7.31
Species: Mouse
PMID: 35620578
Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora
Published: 2021-01-07
Journal: Frontiers in Microbiology
Application: WB
Impact Factor: 5.64
Species: Mouse
PMID: 33519783
Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation
Published: 2020-06-25
Journal: Gut Microbes
Application: WB
Impact Factor: 7.74
Species: Rat
PMID: 32584650
Review
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Product Promise
We promise Anti-LGR5/GPR49 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00239
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026