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Product Info Summary
| SKU: | EK1829 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1829
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Plexin C1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Plxnc1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1829)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A35-T950
Sensitivity
<50 pg/ml
Assay Range
93.8 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1829 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1829 is reactive to PLXNC1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1829 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Plexin C1
A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1829-CAP | Anti-Mouse PLXNC1 Pre-coated 96-well strip microplate | 1 |
| EK1829-ST | Mouse PLXNC1 Standard | 2 vials, 10 ng/tube |
| EK1829-DA | Mouse PLXNC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.066 | 0.166 | 0.190 | 0.301 | 0.617 | 1.038 | 1.642 | 2.421 |
Data Example Images
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Mouse Plexin C1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Plexin C1, Dilution ratio of 1:1, concentration in serum and plasma is around 1200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 168 | 824 | 2614 | 172 | 830 | 2761 |
| Standard deviation | 7.2 | 47.8 | 162.07 | 11.35 | 48.97 | 209.8 |
| CV (%) | 4.3% | 5.8% | 6.2% | 6.6% | 5.9% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Plexin C1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 168 | 149 | 160 | 163 | 160 | 6.96 | 4.3% |
| Sample 2 | 824 | 814 | 878 | 902 | 854 | 36.67 | 4.2% |
| Sample 3 | 2614 | 2932 | 2991 | 2861 | 2849 | 143.54 | 5.0% |
Protein Target Info & Infographic
Gene/Protein Information For PLXNC1 (Source: Uniprot.Org, NCBI)
Gene Name
PLXNC1
Full Name
Plexin-C1
Weight
175.742kDa
Superfamily
plexin family
Alternative Names
CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor PLXNC1 CD232, PLXN-C1, VESPR plexin C1 plexin-C1|plexin (semaphorin receptor)|receptor for viral semaphorin protein|receptor for virally-encoded semaphorin|virus-encoded semaphorin protein receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLXNC1, check out the PLXNC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLXNC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine® (EK1829)
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No publications found for EK1829
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-01-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-12
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-29
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Tiwari
Verified customer
Asked: 2018-12-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-12-09
Question
Q: can you recommend the dilution ratio of serum samples for detection of Plexin C1 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
B. Thomas
Verified customer
Asked: 2016-07-27
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Plexin C1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-07-27
Question
Q: how to analyze ELISA data? I measured Plexin C1 level in plasma.
C. Dhar
Verified customer
Asked: 2015-10-26
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-10-26
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Plexin C1 using the EK1829 Mouse Plexin C1 Picokine® ELISA Kit?
G. Williams
Verified customer
Asked: 2015-08-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2015-08-08
