Mouse Rat Triiodothyronine (T3) Total ELISA Kit

Product Name

Mouse Rat Triiodothyronine (T3) Total ELISA Kit

SKU/Catalog Number

EK7011

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Triiodothyronine (T3). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Rat Triiodothyronine (T3) Total ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7011)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.25 ng/ml

Assay Range

0.25-7.5 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7011 is reactive to in Mouse, Rat samples

Validated Sample Types

Serum

Application Guarantee

EK7011 is guaranteed for ELISA in Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
Microwells coated with T3 Monoclonal Ab	12x8x1 Microwells
T3 Standards	7 vials ( ready to use) 0.25 ml
T3 Enzyme Conjugate concentrate	1 vial 1.5ml
Assay Diluent	1 bottle (ready to use) 12ml
TMB Substrate	1 bottle (ready to use) 12ml
Stop Solution	1 bottle (ready to use) 12ml
20X Wash concentrate	1 bottle 25ml

Standard Concentrations and example data

	OD 450 nm	Conc. ng/mL
Std 1	2.404	0
Std 2	2.238	0.25
Std 3	2.001	0.5
Std 4	1.714	1
Std 5	1.147	2.5
Std 6	0.793	5
Std 7	0.642	7.5

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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