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Product Info Summary
| SKU: | PROTQ04999 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Mouse recombinant Activin B protein, AF
View all Inhbb recombinant proteins
SKU/Catalog Number
PROTQ04999
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Activin B is the cytokine of the TGF beta family, which is a 12.9 kDa protein containing 116 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. It also has a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Mouse recombinant Activin B protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ04999)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
45.2 kDa
Molecular weight
The protein has a calculated MW of 13.71 kDa. The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <1 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MGLECDGRTSLCCRQQFFIDFRLIGWNDWIIAPTGYYGNYCEGSCPAYLAGVPGSASSFHTAVVNQYRMRGLNPGPVNSCCIPTKLSSMSMLYFDDEYNIVKRDVPNMIVEECGCA with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant mouse Activin B
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