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Product Info Summary
| SKU: | PROTQ9JLA2-4 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Mouse recombinant IL-36 alpha (Interleukin-36 alpha) protein, AF
View all Il36a recombinant proteins
SKU/Catalog Number
PROTQ9JLA2-4
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Interleukin 36 alpha (IL-36 alpha) is a 17.76 kDa cytokine with 160 amino acid residues. IL-36 alpha is an IL-1 family member, which binds to the Interleukin-1 receptor and is expressed in the lung, stomach, and placenta. IL-36 alpha is critical in inflammatory responses and stimulates the production of proinflammatory cytokines, such as IL-12, IL-1β, IL-6, and TNF-α. It also induces the generation of IFNγ, IL-4, and IL-17 in CD4+ T cells.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Mouse recombinant IL-36 alpha (Interleukin-36 alpha) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ9JLA2-4)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
18.0 kDa
Molecular weight
The protein has a calculated MW of 18.82 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 alpha is > 1 x 10⁵ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MNKEKELRAASPSLRHVQDLSSRVWILQNNILTAVPRKEQTVPVTITLLPCQYLDTLETNRGDPTYMGVQRPMSCLFCTKDGEQPVLQLGEGNIMEMYNKKEPVKASLFYHKKSGTTSTFESAAFPGWFIAVCSKGSCPLILTQELGEIFITDFEMIVVH with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant mouse IL-36 alpha
Specific Publications For Mouse recombinant IL-36 alpha (Interleukin-36 alpha) protein, AF (PROTQ9JLA2-4)
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