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Product Info Summary
| SKU: | FEK0502 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, citrate). |
| Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse sE-Selectin PicoKine® Quick ELISA Kit
See all E-Selectin/CD62E products
SKU/Catalog Number
FEK0502
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse sE-Selectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Sele in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse sE-Selectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0502)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: W22-P557
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0502 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0502 is reactive to SELE in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, citrate).
Application Guarantee
FEK0502 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of E-Selectin/CD62E
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK0502-CAP | 96-well plate precoated with anti-Mouse E-Selectin/CD62E antibody | 1 |
| EK0502-ST | lyophilized recombinant Mouse E-Selectin/CD62E standard | 20 ng/tube |
| FEK0502-DA | Biotinylated anti-Mouse E-Selectin/CD62E detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.013 | 0.041 | 0.080 | 0.201 | 0.368 | 0.751 | 1.474 | 2.156 |
Data Example Images
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Mouse sE-Selectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1580 | 4329 | 277 | 1451 | 4721 |
| Standard deviation | 11.96 | 105.86 | 186.14 | 16.62 | 108.82 | 250.21 |
| CV (%) | 4.4% | 6.7% | 4.3% | 6% | 7.5% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of E-Selectin/CD62E in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 272 | 280 | 290 | 284 | 281 | 6.53 | 2.3% |
| Sample 2 | 1580 | 1395 | 1401 | 1536 | 1478 | 81.52 | 5.5% |
| Sample 3 | 4329 | 4374 | 4283 | 4168 | 4288 | 76.65 | 1.7% |
Protein Target Info & Infographic
Gene/Protein Information For SELE (Source: Uniprot.Org, NCBI)
Uniprot ID
P16581
Gene ID
6401
Gene Name
SELE
Full Name
E-selectin
Weight
66.655kDa
Superfamily
selectin/LECAM family
Alternative Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E SELE CD62E, ELAM, ELAM1, ESEL, LECAM2 selectin E E-selectin|CD62 antigen-like family member E|ELAM-1|endothelial adhesion molecule 1|endothelial leukocyte adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SELE, check out the SELE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse sE-Selectin PicoKine® Quick ELISA Kit (FEK0502)
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